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Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-4754
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dc.contributor.authorKumar, Pramod-
dc.contributor.authorSatyam, Abhigyan-
dc.contributor.authorFan, Xingliang-
dc.contributor.authorCollin, Estelle-
dc.contributor.authorRochev, Yury-
dc.contributor.authorRodriguez, Brian J.-
dc.contributor.authorGorelov, Alexander-
dc.contributor.authorDillon, Simon-
dc.contributor.authorJoshi, Lokesh-
dc.contributor.authorRaghunath, Michael-
dc.contributor.authorPandit, Abhay-
dc.contributor.authorZeugolis, Dimitrios I.-
dc.date.accessioned2018-10-26T14:18:23Z-
dc.date.available2018-10-26T14:18:23Z-
dc.date.issued2015-
dc.identifier.issn2045-2322de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/12199-
dc.description.abstractTherapeutic strategies based on the principles of tissue engineering by self-assembly put forward the notion that functional regeneration can be achieved by utilising the inherent capacity of cells to create highly sophisticated supramolecular assemblies. However, in dilute ex-vivo microenvironments, prolonged culture time is required to develop an extracellular matrix-rich implantable device. Herein, we assessed the influence of macromolecular crowding, a biophysical phenomenon that regulates intra- and extra-cellular activities in multicellular organisms, in human corneal fibroblast culture. In the presence of macromolecules, abundant extracellular matrix deposition was evidenced as fast as 48 h in culture, even at low serum concentration. Temperature responsive copolymers allowed the detachment of dense and cohesive supramolecularly assembled living substitutes within 6 days in culture. Morphological, histological, gene and protein analysis assays demonstrated maintenance of tissue-specific function. Macromolecular crowding opens new avenues for a more rational design in engineering of clinically relevant tissue modules in vitro.de_CH
dc.language.isoende_CH
dc.publisherNature Publishing Groupde_CH
dc.relation.ispartofScientific Reportsde_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subject.ddc571: Physiologie und verwandte Themende_CH
dc.subject.ddc572: Biochemiede_CH
dc.titleMacromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assembliesde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.21256/zhaw-4754-
dc.identifier.doi10.1038/srep08729de_CH
zhaw.funding.euNode_CH
zhaw.issue8729de_CH
zhaw.originated.zhawNode_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume5de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedMetabolic Tissue Engineeringde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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Kumar, P., Satyam, A., Fan, X., Collin, E., Rochev, Y., Rodriguez, B. J., Gorelov, A., Dillon, S., Joshi, L., Raghunath, M., Pandit, A., & Zeugolis, D. I. (2015). Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies. Scientific Reports, 5(8729). https://doi.org/10.21256/zhaw-4754
Kumar, P. et al. (2015) ‘Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies’, Scientific Reports, 5(8729). Available at: https://doi.org/10.21256/zhaw-4754.
P. Kumar et al., “Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies,” Scientific Reports, vol. 5, no. 8729, 2015, doi: 10.21256/zhaw-4754.
KUMAR, Pramod, Abhigyan SATYAM, Xingliang FAN, Estelle COLLIN, Yury ROCHEV, Brian J. RODRIGUEZ, Alexander GORELOV, Simon DILLON, Lokesh JOSHI, Michael RAGHUNATH, Abhay PANDIT und Dimitrios I. ZEUGOLIS, 2015. Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies. Scientific Reports. 2015. Bd. 5, Nr. 8729. DOI 10.21256/zhaw-4754
Kumar, Pramod, Abhigyan Satyam, Xingliang Fan, Estelle Collin, Yury Rochev, Brian J. Rodriguez, Alexander Gorelov, et al. 2015. “Macromolecularly Crowded in Vitro Microenvironments Accelerate the Production of Extracellular Matrix-Rich Supramolecular Assemblies.” Scientific Reports 5 (8729). https://doi.org/10.21256/zhaw-4754.
Kumar, Pramod, et al. “Macromolecularly Crowded in Vitro Microenvironments Accelerate the Production of Extracellular Matrix-Rich Supramolecular Assemblies.” Scientific Reports, vol. 5, no. 8729, 2015, https://doi.org/10.21256/zhaw-4754.


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