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|Title:||Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies|
|Authors :||Kumar, Pramod|
Rodriguez, Brian J.
Zeugolis, Dimitrios I.
|Published in :||Scientific Reports|
|Publisher / Ed. Institution :||Nature Publishing Group|
|License (according to publishing contract) :||CC BY 4.0: Attribution 4.0 International|
|Type of review:||Peer review (publication)|
|Subject (DDC) :||571: Physiology and related subjects |
|Abstract:||Therapeutic strategies based on the principles of tissue engineering by self-assembly put forward the notion that functional regeneration can be achieved by utilising the inherent capacity of cells to create highly sophisticated supramolecular assemblies. However, in dilute ex-vivo microenvironments, prolonged culture time is required to develop an extracellular matrix-rich implantable device. Herein, we assessed the influence of macromolecular crowding, a biophysical phenomenon that regulates intra- and extra-cellular activities in multicellular organisms, in human corneal fibroblast culture. In the presence of macromolecules, abundant extracellular matrix deposition was evidenced as fast as 48 h in culture, even at low serum concentration. Temperature responsive copolymers allowed the detachment of dense and cohesive supramolecularly assembled living substitutes within 6 days in culture. Morphological, histological, gene and protein analysis assays demonstrated maintenance of tissue-specific function. Macromolecular crowding opens new avenues for a more rational design in engineering of clinically relevant tissue modules in vitro.|
|Departement:||Life Sciences and Facility Management|
|Organisational Unit:||Institute of Chemistry and Biotechnology (ICBT)|
|Publication type:||Article in scientific journal|
|Appears in Collections:||Publikationen Life Sciences und Facility Management|
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|2015_Kumar_et_al_Macromolecularity_crowded_in_vitro_microenvironments_srep08729.pdf||4.53 MB||Adobe PDF|
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