| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Palmitoylation of the three isoforms of human endothelin-converting enzyme-1 |
| Autor/-in: | Schweizer, Anja Löffler, Bernd-Michael Rohrer, Jack |
| DOI: | 10.1042/bj3400649 |
| Erschienen in: | Biochemical Journal |
| Band(Heft): | 340 |
| Heft: | 3 |
| Seite(n): | 649 |
| Seiten bis: | 656 |
| Erscheinungsdatum: | 15-Jun-1999 |
| Verlag / Hrsg. Institution: | Portland Press |
| ISSN: | 0264-6021 |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 572: Biochemie |
| Zusammenfassung: | Endothelin-converting enzyme-1 (ECE-1) is a membrane-bound metalloprotease that catalyses the conversion of inactive big endothelins into active endothelins. Here we have examined whether the three isoforms of human ECE-1 (ECE-1a, ECE-1b and ECE-1c) are modified by the covalent attachment of the fatty acid palmitate and have evaluated a potential functional role of this modification. To do this, wild-type and mutant enzymes were expressed and analysed by metabolic labelling with [3H]palmitate, immunoprecipitation and SDS/PAGE. All three ECE-1 isoforms were found to be palmitoylated via hydroxylamine-sensitive thioester bonds. In addition, the isoforms showed similar levels of acylation. Cys46 in ECE-1a, Cys58 in ECE-1b and Cys42 in ECE-1c were identified as sites of palmitoylation and each of these cysteines accounted for all the palmitoylation that occurred in the corresponding isoform. Immunofluorescence analysis demonstrated further that palmitoylated and non-palmitoylated ECE-1 isoforms had the same subcellular localizations. Moreover, complete solubility of the three isoforms in Triton X-100 revealed that palmitoylation does not target ECE-1 to cholesterol and sphingolipid-rich membrane domains or caveolae. The enzymic activities of ECE-1a, ECE-1b and ECE-1c were also not significantly affected by the absence of palmitoylation. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/6848 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC1220295&blobtype=pdf |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Schweizer, A., Löffler, B.-M., & Rohrer, J. (1999). Palmitoylation of the three isoforms of human endothelin-converting enzyme-1. Biochemical Journal, 340(3), 649–656. https://doi.org/10.1042/bj3400649
Schweizer, A., Löffler, B.-M. and Rohrer, J. (1999) ‘Palmitoylation of the three isoforms of human endothelin-converting enzyme-1’, Biochemical Journal, 340(3), pp. 649–656. Available at: https://doi.org/10.1042/bj3400649.
A. Schweizer, B.-M. Löffler, and J. Rohrer, “Palmitoylation of the three isoforms of human endothelin-converting enzyme-1,” Biochemical Journal, vol. 340, no. 3, pp. 649–656, Jun. 1999, doi: 10.1042/bj3400649.
SCHWEIZER, Anja, Bernd-Michael LÖFFLER und Jack ROHRER, 1999. Palmitoylation of the three isoforms of human endothelin-converting enzyme-1. Biochemical Journal [online]. 15 Juni 1999. Bd. 340, Nr. 3, S. 649–656. DOI 10.1042/bj3400649. Verfügbar unter: http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC1220295&blobtype=pdf
Schweizer, Anja, Bernd-Michael Löffler, and Jack Rohrer. 1999. “Palmitoylation of the Three Isoforms of Human Endothelin-Converting Enzyme-1.” Biochemical Journal 340 (3): 649–56. https://doi.org/10.1042/bj3400649.
Schweizer, Anja, et al. “Palmitoylation of the Three Isoforms of Human Endothelin-Converting Enzyme-1.” Biochemical Journal, vol. 340, no. 3, June 1999, pp. 649–56, https://doi.org/10.1042/bj3400649.
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