Palmitoylation of the three isoforms of human endothelin-converting enzyme-1

Schweizer, Anja; Löffler, Bernd-Michael; Rohrer, Jack

doi:10.1042/bj3400649

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Palmitoylation of the three isoforms of human endothelin-converting enzyme-1
Authors:	Schweizer, Anja Löffler, Bernd-Michael Rohrer, Jack
DOI:	10.1042/bj3400649
Published in:	Biochemical Journal
Volume(Issue):	340
Issue:	3
Page(s):	649
Pages to:	656
Issue Date:	15-Jun-1999
Publisher / Ed. Institution:	Portland Press
ISSN:	0264-6021
Language:	English
Subject (DDC):	572: Biochemistry
Abstract:	Endothelin-converting enzyme-1 (ECE-1) is a membrane-bound metalloprotease that catalyses the conversion of inactive big endothelins into active endothelins. Here we have examined whether the three isoforms of human ECE-1 (ECE-1a, ECE-1b and ECE-1c) are modified by the covalent attachment of the fatty acid palmitate and have evaluated a potential functional role of this modification. To do this, wild-type and mutant enzymes were expressed and analysed by metabolic labelling with [3H]palmitate, immunoprecipitation and SDS/PAGE. All three ECE-1 isoforms were found to be palmitoylated via hydroxylamine-sensitive thioester bonds. In addition, the isoforms showed similar levels of acylation. Cys46 in ECE-1a, Cys58 in ECE-1b and Cys42 in ECE-1c were identified as sites of palmitoylation and each of these cysteines accounted for all the palmitoylation that occurred in the corresponding isoform. Immunofluorescence analysis demonstrated further that palmitoylated and non-palmitoylated ECE-1 isoforms had the same subcellular localizations. Moreover, complete solubility of the three isoforms in Triton X-100 revealed that palmitoylation does not target ECE-1 to cholesterol and sphingolipid-rich membrane domains or caveolae. The enzymic activities of ECE-1a, ECE-1b and ECE-1c were also not significantly affected by the absence of palmitoylation.
URI:	https://digitalcollection.zhaw.ch/handle/11475/6848 http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC1220295&blobtype=pdf
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Appears in collections:	Publikationen Life Sciences und Facility Management

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Schweizer, A., Löffler, B.-M., & Rohrer, J. (1999). Palmitoylation of the three isoforms of human endothelin-converting enzyme-1. Biochemical Journal, 340(3), 649–656. https://doi.org/10.1042/bj3400649

Schweizer, A., Löffler, B.-M. and Rohrer, J. (1999) ‘Palmitoylation of the three isoforms of human endothelin-converting enzyme-1’, Biochemical Journal, 340(3), pp. 649–656. Available at: https://doi.org/10.1042/bj3400649.

A. Schweizer, B.-M. Löffler, and J. Rohrer, “Palmitoylation of the three isoforms of human endothelin-converting enzyme-1,” Biochemical Journal, vol. 340, no. 3, pp. 649–656, Jun. 1999, doi: 10.1042/bj3400649.

SCHWEIZER, Anja, Bernd-Michael LÖFFLER und Jack ROHRER, 1999. Palmitoylation of the three isoforms of human endothelin-converting enzyme-1. Biochemical Journal [online]. 15 Juni 1999. Bd. 340, Nr. 3, S. 649–656. DOI 10.1042/bj3400649. Verfügbar unter: http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC1220295&blobtype=pdf

Schweizer, Anja, Bernd-Michael Löffler, and Jack Rohrer. 1999. “Palmitoylation of the Three Isoforms of Human Endothelin-Converting Enzyme-1.” Biochemical Journal 340 (3): 649–56. https://doi.org/10.1042/bj3400649.

Schweizer, Anja, et al. “Palmitoylation of the Three Isoforms of Human Endothelin-Converting Enzyme-1.” Biochemical Journal, vol. 340, no. 3, June 1999, pp. 649–56, https://doi.org/10.1042/bj3400649.