| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Keine Angabe |
| Titel: | Engineering fire blight resistance into the apple cultivar 'Gala' using the FB_MR5 CC-NBS-LRR resistance gene of Malus × robusta 5 Giovanni |
| Autor/-in: | Broggini, Giovanni A. L. Wöhner, Thomas Fahrentrapp, Johannes Kost, Thomas D. Flachowsky, Henryk Peil, Andreas Hanke, Maria-Viola Richter, Klaus Patocchi, Andrea Gessler, Cesare |
| DOI: | 10.1111/pbi.12177 |
| Erschienen in: | Plant Biotechnology Journal |
| Band(Heft): | 12 |
| Heft: | 6 |
| Seite(n): | 728 |
| Seiten bis: | 733 |
| Erscheinungsdatum: | Mär-2014 |
| Verlag / Hrsg. Institution: | Wiley |
| ISSN: | 1467-7644 1467-7652 |
| Sprache: | Englisch |
| Schlagwörter: | Genetic engineering; GMO; Resistance; Weinbau |
| Fachgebiet (DDC): | 634: Obstanlagen, Früchte und Forstwirtschaft 660.6: Biotechnologie |
| Zusammenfassung: | The fire blight susceptible apple cultivar Malus × domestica Borkh. cv. ‘Gala’ was transformed with the candidate fire blight resistance gene FB_MR5 originating from the crab apple accession Malus × robusta 5 (Mr5). A total of five different transgenic lines were obtained. All transgenic lines were shown to be stably transformed and originate from different transgenic events. The transgenic lines express the FB_MR5 either driven by the constitutive CaMV 35S promoter and the ocs terminator or by its native promoter and terminator sequences. Phenotyping experiments were performed with Mr5-virulent and Mr5-avirulent strains of Erwinia amylovora, the causal agent of fire blight. Significantly less disease symptoms were detected on transgenic lines after inoculation with two different Mr5-avirulent E. amylovora strains, while significantly more shoot necrosis was observed after inoculation with the Mr5-virulent mutant strain ZYRKD3_1. The results of these experiments demonstrated the ability of a single gene isolated from the native gene pool of apple to protect a susceptible cultivar from fire blight. Furthermore, this gene is confirmed to be the resistance determinant of Mr5 as the transformed lines undergo the same gene-for-gene interaction in the host–pathogen relationship Mr5–E. amylovora. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/4095 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Umwelt und Natürliche Ressourcen (IUNR) |
| Publiziert im Rahmen des ZHAW-Projekts: | Occurence of grape phylloxera (Dactulosphaira vitifoliae) in Switzerland |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Broggini, G. A. L., Wöhner, T., Fahrentrapp, J., Kost, T. D., Flachowsky, H., Peil, A., Hanke, M.-V., Richter, K., Patocchi, A., & Gessler, C. (2014). Engineering fire blight resistance into the apple cultivar ‘Gala’ using the FB_MR5 CC-NBS-LRR resistance gene of Malus × robusta 5 Giovanni. Plant Biotechnology Journal, 12(6), 728–733. https://doi.org/10.1111/pbi.12177
Broggini, G.A.L. et al. (2014) ‘Engineering fire blight resistance into the apple cultivar “Gala” using the FB_MR5 CC-NBS-LRR resistance gene of Malus × robusta 5 Giovanni’, Plant Biotechnology Journal, 12(6), pp. 728–733. Available at: https://doi.org/10.1111/pbi.12177.
G. A. L. Broggini et al., “Engineering fire blight resistance into the apple cultivar ‘Gala’ using the FB_MR5 CC-NBS-LRR resistance gene of Malus × robusta 5 Giovanni,” Plant Biotechnology Journal, vol. 12, no. 6, pp. 728–733, Mar. 2014, doi: 10.1111/pbi.12177.
BROGGINI, Giovanni A. L., Thomas WÖHNER, Johannes FAHRENTRAPP, Thomas D. KOST, Henryk FLACHOWSKY, Andreas PEIL, Maria-Viola HANKE, Klaus RICHTER, Andrea PATOCCHI und Cesare GESSLER, 2014. Engineering fire blight resistance into the apple cultivar ‚Gala‘ using the FB_MR5 CC-NBS-LRR resistance gene of Malus × robusta 5 Giovanni. Plant Biotechnology Journal. März 2014. Bd. 12, Nr. 6, S. 728–733. DOI 10.1111/pbi.12177
Broggini, Giovanni A. L., Thomas Wöhner, Johannes Fahrentrapp, Thomas D. Kost, Henryk Flachowsky, Andreas Peil, Maria-Viola Hanke, Klaus Richter, Andrea Patocchi, and Cesare Gessler. 2014. “Engineering Fire Blight Resistance into the Apple Cultivar ‘Gala’ Using the FB_MR5 CC-NBS-LRR Resistance Gene of Malus × Robusta 5 Giovanni.” Plant Biotechnology Journal 12 (6): 728–33. https://doi.org/10.1111/pbi.12177.
Broggini, Giovanni A. L., et al. “Engineering Fire Blight Resistance into the Apple Cultivar ‘Gala’ Using the FB_MR5 CC-NBS-LRR Resistance Gene of Malus × Robusta 5 Giovanni.” Plant Biotechnology Journal, vol. 12, no. 6, Mar. 2014, pp. 728–33, https://doi.org/10.1111/pbi.12177.
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