Title: Engineering fire blight resistance into the apple cultivar 'Gala' using the FB_MR5 CC-NBS-LRR resistance gene of Malus × robusta 5 Giovanni
Authors : Broggini, Giovanni A. L.
Wöhner, Thomas
Fahrentrapp, Johannes
Kost, Thomas D.
Flachowsky, Henryk
Peil, Andreas
Hanke, Maria-Viola
Richter, Klaus
Patocchi, Andrea
Gessler, Cesare
Published in : Plant Biotechnology Journal
Volume(Issue) : 12
Issue : 6
Pages : 728
Pages to: 733
Publisher / Ed. Institution : Wiley-Blackwell
Issue Date: Mar-2014
Language : Englisch / English
Subjects : Genetic engineering; GMO; Resistance; Weinbau
Subject (DDC) : 634: Obstanlagen, Früchte, Forstwirtschaft
660.6: Biotechnologie
Abstract: The fire blight susceptible apple cultivar Malus × domestica Borkh. cv. ‘Gala’ was transformed with the candidate fire blight resistance gene FB_MR5 originating from the crab apple accession Malus × robusta 5 (Mr5). A total of five different transgenic lines were obtained. All transgenic lines were shown to be stably transformed and originate from different transgenic events. The transgenic lines express the FB_MR5 either driven by the constitutive CaMV 35S promoter and the ocs terminator or by its native promoter and terminator sequences. Phenotyping experiments were performed with Mr5-virulent and Mr5-avirulent strains of Erwinia amylovora, the causal agent of fire blight. Significantly less disease symptoms were detected on transgenic lines after inoculation with two different Mr5-avirulent E. amylovora strains, while significantly more shoot necrosis was observed after inoculation with the Mr5-virulent mutant strain ZYRKD3_1. The results of these experiments demonstrated the ability of a single gene isolated from the native gene pool of apple to protect a susceptible cultivar from fire blight. Furthermore, this gene is confirmed to be the resistance determinant of Mr5 as the transformed lines undergo the same gene-for-gene interaction in the host–pathogen relationship Mr5–E. amylovora.
Departement: Life Sciences und Facility Management
Organisational Unit: Institut für Umwelt und Natürliche Ressourcen (IUNR)
Publication type: Beitrag in wissenschaftlicher Zeitschrift / Article in scientific Journal
Type of review: Keine Angabe / Not specified
DOI : 10.1111/pbi.12177
ISSN: 1467-7644
URI: https://digitalcollection.zhaw.ch/handle/11475/4095
License (according to publishing contract) : Lizenz gemäss Verlagsvertrag / Licence according to publishing contract
Published as part of the ZHAW project : Occurence of grape phylloxera (Dactulosphaira vitifoliae) in Switzerland
Appears in Collections:Publikationen Life Sciences und Facility Management

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