Title: Microspectroscopic analysis of green fluorescent proteins infiltrated into mesoporous silica nanochannels
Authors : Ma, Yujie
Rajendran, Prayanka
Blum, Christian
Cesa, Yanina
Gartmann, Nando
Brühwiler, Dominik
Subramaniam, Vinod
Published in : Journal of Colloid and Interface Science
Volume(Issue) : 356
Issue : 1
Pages : 123
Pages to: 130
Publisher / Ed. Institution : Academic Press
Issue Date: 1-Apr-2011
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subjects : ICBC; Mesopores; Proteins
Subject (DDC) : 540: Chemistry
Abstract: The infiltration of enhanced green fluorescent protein (EGFP) into nanochannels of different diameters in mesoporous silica particles was studied in detail by fluorescence microspectroscopy at room temperature. Silica particles from the MCM-41, ASNCs and SBA-15 families possessing nanometer-sized (3–8 nm in diameter) channels, comparable to the dimensions of the infiltrated guest protein EGFP (barrel structure with dimensions of 2.4 nm × 4.2 nm), were used as hosts. We found that it is necessary to first functionalize the surfaces of the silica particles with an amino-silane for effective encapsulation of EGFP. We demonstrated successful infiltration of the protein into the nanochannels based on fluorescence microspectroscopy and loading capacity calculations, even for nanochannel diameters approaching the protein dimensions. We studied the spatial distributions of the EGFPs within the silica particles by confocal laser scanning microscopy (CLSM) and multimode microscopy. Upon infiltration, the fluorescence lifetime drops as expected for an emitter embedded in a high refractive index medium. Further, the spectral properties of EGFP are preserved, confirming the structural integrity of the infiltrated protein. This inorganic-protein host-guest system is an example of a nanobiophotonic hybrid system that may lead to composite materials with novel optical properties.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific Journal
DOI : 10.1016/j.jcis.2010.12.082
ISSN: 0021-9797
URI: https://digitalcollection.zhaw.ch/handle/11475/2676
Appears in Collections:Publikationen Life Sciences und Facility Management

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