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https://doi.org/10.21256/zhaw-26098| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Enhancing the efficacy of stem cell therapy with glycosaminoglycans |
| Autor/-in: | Ling, Ling Ren, Xiafei Cao, Xue Hassan, Afizah Binte Mohd Mah, Sophia Sathiyanathan, Padmapriya Smith, Raymond A. A. Tan, Clarissa L. L. Eio, Michelle Samsonraj, Rebekah M. van Wijnen, Andre J. Raghunath, Michael Nurcombe, Victor Hui, James H. Cool, Simon M. |
| et. al: | No |
| DOI: | 10.1016/j.stemcr.2019.12.003 10.21256/zhaw-26098 |
| Erschienen in: | Stem Cell Reports |
| Band(Heft): | 14 |
| Heft: | 1 |
| Seite(n): | 105 |
| Seiten bis: | 121 |
| Erscheinungsdatum: | 2020 |
| Verlag / Hrsg. Institution: | Cell Press |
| ISSN: | 2213-6711 |
| Sprache: | Englisch |
| Schlagwörter: | Heparan sulfate; Stemness; Ex vivo expansion; FGF2; Osteochondral regeneration; Cartilage repair; Efficacy; Potency |
| Fachgebiet (DDC): | 616: Innere Medizin und Krankheiten |
| Zusammenfassung: | Human mesenchymal stem cell (hMSC) therapy offers significant potential for osteochondral regeneration. Such applications require their ex vivo expansion in media frequently supplemented with fibroblast growth factor 2 (FGF2). Particular heparan sulfate (HS) fractions stabilize FGF2-FGF receptor complexes. We show that an FGF2-binding HS variant (HS8) accelerates the expansion of freshly isolated bone marrow hMSCs without compromising their naivety. Importantly, the repair of osteochondral defects in both rats and pigs is improved after treatment with HS8-supplemented hMSCs (MSCHS8), when assessed histologically, biomechanically, or by MRI. Thus, supplementing hMSC culture media with an HS variant that targets endogenously produced FGF2 allows the elimination of exogenous growth factors that may adversely affect their therapeutic potency. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/26098 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | CC BY-NC-ND 4.0: Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
Dateien zu dieser Ressource:
| Datei | Beschreibung | Größe | Format | |
|---|---|---|---|---|
| 2020_Ling-etal_Enhancing-efficacy-stem-cell-therapy-glycosaminoglycans_StemCellReports.pdf | 11.94 MB | Adobe PDF |  Öffnen/Anzeigen |
Zur Langanzeige
Ling, L., Ren, X., Cao, X., Hassan, A. B. M., Mah, S., Sathiyanathan, P., Smith, R. A. A., Tan, C. L. L., Eio, M., Samsonraj, R. M., van Wijnen, A. J., Raghunath, M., Nurcombe, V., Hui, J. H., & Cool, S. M. (2020). Enhancing the efficacy of stem cell therapy with glycosaminoglycans. Stem Cell Reports, 14(1), 105–121. https://doi.org/10.1016/j.stemcr.2019.12.003
Ling, L. et al. (2020) ‘Enhancing the efficacy of stem cell therapy with glycosaminoglycans’, Stem Cell Reports, 14(1), pp. 105–121. Available at: https://doi.org/10.1016/j.stemcr.2019.12.003.
L. Ling et al., “Enhancing the efficacy of stem cell therapy with glycosaminoglycans,” Stem Cell Reports, vol. 14, no. 1, pp. 105–121, 2020, doi: 10.1016/j.stemcr.2019.12.003.
LING, Ling, Xiafei REN, Xue CAO, Afizah Binte Mohd HASSAN, Sophia MAH, Padmapriya SATHIYANATHAN, Raymond A. A. SMITH, Clarissa L. L. TAN, Michelle EIO, Rebekah M. SAMSONRAJ, Andre J. VAN WIJNEN, Michael RAGHUNATH, Victor NURCOMBE, James H. HUI und Simon M. COOL, 2020. Enhancing the efficacy of stem cell therapy with glycosaminoglycans. Stem Cell Reports. 2020. Bd. 14, Nr. 1, S. 105–121. DOI 10.1016/j.stemcr.2019.12.003
Ling, Ling, Xiafei Ren, Xue Cao, Afizah Binte Mohd Hassan, Sophia Mah, Padmapriya Sathiyanathan, Raymond A. A. Smith, et al. 2020. “Enhancing the Efficacy of Stem Cell Therapy with Glycosaminoglycans.” Stem Cell Reports 14 (1): 105–21. https://doi.org/10.1016/j.stemcr.2019.12.003.
Ling, Ling, et al. “Enhancing the Efficacy of Stem Cell Therapy with Glycosaminoglycans.” Stem Cell Reports, vol. 14, no. 1, 2020, pp. 105–21, https://doi.org/10.1016/j.stemcr.2019.12.003.
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