Enhancing the efficacy of stem cell therapy with glycosaminoglycans

Ling, Ling; Ren, Xiafei; Cao, Xue; Hassan, Afizah Binte Mohd; Mah, Sophia; Sathiyanathan, Padmapriya; Smith, Raymond A. A.; Tan, Clarissa L. L.; Eio, Michelle; Samsonraj, Rebekah M.; van Wijnen, Andre J.; Raghunath, Michael; Nurcombe, Victor; Hui, James H.; Cool, Simon M.

doi:10.1016/j.stemcr.2019.12.003

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-26098

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Enhancing the efficacy of stem cell therapy with glycosaminoglycans
Authors:	Ling, Ling Ren, Xiafei Cao, Xue Hassan, Afizah Binte Mohd Mah, Sophia Sathiyanathan, Padmapriya Smith, Raymond A. A. Tan, Clarissa L. L. Eio, Michelle Samsonraj, Rebekah M. van Wijnen, Andre J. Raghunath, Michael Nurcombe, Victor Hui, James H. Cool, Simon M.
et. al:	No
DOI:	10.1016/j.stemcr.2019.12.003 10.21256/zhaw-26098
Published in:	Stem Cell Reports
Volume(Issue):	14
Issue:	1
Page(s):	105
Pages to:	121
Issue Date:	2020
Publisher / Ed. Institution:	Cell Press
ISSN:	2213-6711
Language:	English
Subjects:	Heparan sulfate; Stemness; Ex vivo expansion; FGF2; Osteochondral regeneration; Cartilage repair; Efficacy; Potency
Subject (DDC):	616: Internal medicine and diseases
Abstract:	Human mesenchymal stem cell (hMSC) therapy offers significant potential for osteochondral regeneration. Such applications require their ex vivo expansion in media frequently supplemented with fibroblast growth factor 2 (FGF2). Particular heparan sulfate (HS) fractions stabilize FGF2-FGF receptor complexes. We show that an FGF2-binding HS variant (HS8) accelerates the expansion of freshly isolated bone marrow hMSCs without compromising their naivety. Importantly, the repair of osteochondral defects in both rats and pigs is improved after treatment with HS8-supplemented hMSCs (MSCHS8), when assessed histologically, biomechanically, or by MRI. Thus, supplementing hMSC culture media with an HS variant that targets endogenously produced FGF2 allows the elimination of exogenous growth factors that may adversely affect their therapeutic potency.
URI:	https://digitalcollection.zhaw.ch/handle/11475/26098
Fulltext version:	Published version
License (according to publishing contract):	CC BY-NC-ND 4.0: Attribution - Non commercial - No derivatives 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Ling, L., Ren, X., Cao, X., Hassan, A. B. M., Mah, S., Sathiyanathan, P., Smith, R. A. A., Tan, C. L. L., Eio, M., Samsonraj, R. M., van Wijnen, A. J., Raghunath, M., Nurcombe, V., Hui, J. H., & Cool, S. M. (2020). Enhancing the efficacy of stem cell therapy with glycosaminoglycans. Stem Cell Reports, 14(1), 105–121. https://doi.org/10.1016/j.stemcr.2019.12.003

Ling, L. et al. (2020) ‘Enhancing the efficacy of stem cell therapy with glycosaminoglycans’, Stem Cell Reports, 14(1), pp. 105–121. Available at: https://doi.org/10.1016/j.stemcr.2019.12.003.

L. Ling et al., “Enhancing the efficacy of stem cell therapy with glycosaminoglycans,” Stem Cell Reports, vol. 14, no. 1, pp. 105–121, 2020, doi: 10.1016/j.stemcr.2019.12.003.

LING, Ling, Xiafei REN, Xue CAO, Afizah Binte Mohd HASSAN, Sophia MAH, Padmapriya SATHIYANATHAN, Raymond A. A. SMITH, Clarissa L. L. TAN, Michelle EIO, Rebekah M. SAMSONRAJ, Andre J. VAN WIJNEN, Michael RAGHUNATH, Victor NURCOMBE, James H. HUI und Simon M. COOL, 2020. Enhancing the efficacy of stem cell therapy with glycosaminoglycans. Stem Cell Reports. 2020. Bd. 14, Nr. 1, S. 105–121. DOI 10.1016/j.stemcr.2019.12.003

Ling, Ling, Xiafei Ren, Xue Cao, Afizah Binte Mohd Hassan, Sophia Mah, Padmapriya Sathiyanathan, Raymond A. A. Smith, et al. 2020. “Enhancing the Efficacy of Stem Cell Therapy with Glycosaminoglycans.” Stem Cell Reports 14 (1): 105–21. https://doi.org/10.1016/j.stemcr.2019.12.003.

Ling, Ling, et al. “Enhancing the Efficacy of Stem Cell Therapy with Glycosaminoglycans.” Stem Cell Reports, vol. 14, no. 1, 2020, pp. 105–21, https://doi.org/10.1016/j.stemcr.2019.12.003.