| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Microarrays made easy : biofunctionalized hydrogel channels for rapid protein microarray production |
| Autor/-in: | de Lange, Victoria Binkert, Andreas Vörös, Janos Bally, Marta |
| DOI: | 10.1021/am100849f |
| Erschienen in: | ACS Applied Materials & Interfaces |
| Band(Heft): | 3 |
| Heft: | 1 |
| Seite(n): | 50 |
| Seiten bis: | 57 |
| Erscheinungsdatum: | 2011 |
| Verlag / Hrsg. Institution: | American Chemical Society |
| ISSN: | 1944-8244 1944-8252 |
| Sprache: | Englisch |
| Schlagwörter: | Human; Hydrogel; Immunoglobulin G; Microarray Analysis; Protein Array Analysis; Time Factor; Polyethylene Glycol Dimethacrylate |
| Fachgebiet (DDC): | 540: Chemie |
| Zusammenfassung: | We present a simple, inexpensive, and sensitive technique for producing multiple copies of a hydrogel-based protein microarray. An agarose block containing 25 biofunctionalized channels is sliced perpendicularly to produce many identical biochips. Each microarray consists of 500 μm spots, which contain protein-coated microparticles physically trapped in porous SeaPrep agarose. Proteins diffuse readily through SeaPrep agarose, while the larger microparticles are immobilized in the hydrogel matrix. Without major assay optimization, the limit of detection is 12 pM for a sandwich assay detecting human IgG. These highly flexible, multiplexed arrays can be produced rapidly without any special instrumentation and are compatible with standard fluorescence-based read-out. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/1732 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | School of Engineering |
| Enthalten in den Sammlungen: | Publikationen School of Engineering |
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de Lange, V., Binkert, A., Vörös, J., & Bally, M. (2011). Microarrays made easy : biofunctionalized hydrogel channels for rapid protein microarray production. ACS Applied Materials & Interfaces, 3(1), 50–57. https://doi.org/10.1021/am100849f
de Lange, V. et al. (2011) ‘Microarrays made easy : biofunctionalized hydrogel channels for rapid protein microarray production’, ACS Applied Materials & Interfaces, 3(1), pp. 50–57. Available at: https://doi.org/10.1021/am100849f.
V. de Lange, A. Binkert, J. Vörös, and M. Bally, “Microarrays made easy : biofunctionalized hydrogel channels for rapid protein microarray production,” ACS Applied Materials & Interfaces, vol. 3, no. 1, pp. 50–57, 2011, doi: 10.1021/am100849f.
DE LANGE, Victoria, Andreas BINKERT, Janos VÖRÖS und Marta BALLY, 2011. Microarrays made easy : biofunctionalized hydrogel channels for rapid protein microarray production. ACS Applied Materials & Interfaces. 2011. Bd. 3, Nr. 1, S. 50–57. DOI 10.1021/am100849f
de Lange, Victoria, Andreas Binkert, Janos Vörös, and Marta Bally. 2011. “Microarrays Made Easy : Biofunctionalized Hydrogel Channels for Rapid Protein Microarray Production.” ACS Applied Materials & Interfaces 3 (1): 50–57. https://doi.org/10.1021/am100849f.
de Lange, Victoria, et al. “Microarrays Made Easy : Biofunctionalized Hydrogel Channels for Rapid Protein Microarray Production.” ACS Applied Materials & Interfaces, vol. 3, no. 1, 2011, pp. 50–57, https://doi.org/10.1021/am100849f.
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