Title: Microarrays made easy : biofunctionalized hydrogel channels for rapid protein microarray production
Authors : de Lange, Victoria
Binkert, Andreas
Vörös, Janos
Bally, Marta
Published in : ACS Applied Materials & Interfaces
Volume(Issue) : 3
Issue : 1
Pages : 50
Pages to: 57
Publisher / Ed. Institution : American Chemical Society
Issue Date: 2011
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subjects : Human; Hydrogel; Immunoglobulin G; Microarray Analysis; Protein Array Analysis; Time Factor; Polyethylene Glycol Dimethacrylate
Subject (DDC) : 540: Chemistry
Abstract: We present a simple, inexpensive, and sensitive technique for producing multiple copies of a hydrogel-based protein microarray. An agarose block containing 25 biofunctionalized channels is sliced perpendicularly to produce many identical biochips. Each microarray consists of 500 μm spots, which contain protein-coated microparticles physically trapped in porous SeaPrep agarose. Proteins diffuse readily through SeaPrep agarose, while the larger microparticles are immobilized in the hydrogel matrix. Without major assay optimization, the limit of detection is 12 pM for a sandwich assay detecting human IgG. These highly flexible, multiplexed arrays can be produced rapidly without any special instrumentation and are compatible with standard fluorescence-based read-out.
Departement: School of Engineering
Publication type: Article in scientific Journal
DOI : 10.1021/am100849f
ISSN: 1944-8244
URI: https://digitalcollection.zhaw.ch/handle/11475/1732
Appears in Collections:Publikationen School of Engineering

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