| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | In vitro evaluation of Ficoll‐enriched and genipin‐stabilised collagen scaffolds |
| Autor/-in: | Satyam, A. Subramanian, G. S. Raghunath, M. Pandit, A. Zeugolis, D. I. |
| DOI: | 10.1002/term.1522 |
| Erschienen in: | Journal of Tissue Engineering and Regenerative Medicine |
| Band(Heft): | 8 |
| Heft: | 3 |
| Seite(n): | 233 |
| Seiten bis: | 241 |
| Erscheinungsdatum: | Mai-2012 |
| Verlag / Hrsg. Institution: | Wiley |
| ISSN: | 1932-7005 |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 572: Biochemie 610: Medizin und Gesundheit |
| Zusammenfassung: | Polysaccharides are frequently incorporated into scaffolds for tissue engineering applications to improve mechanical and biological properties. We evaluated the influence of a Ficoll® scaffold on collagen films, a scaffold that is extensively used for soft and hard tissue repair. To avoid cytotoxicity issues associated with chemical reagents, the influence of genipin, a naturally occurring crosslinking agent, was assessed. Ultra‐structural level collagen films formed with and without Ficoll showed a fine fibrillar structure whereas genipin crosslinked films showed a coarse fibrillar and partially nodular structure. In contrast, glutaraldehyde crosslinked films lost their fibrillar pattern. Crosslinking significantly increased denaturation temperature (p < 0.001), stress (p < 0.0001) and force (p < 0.0001) at break. Collagen/Ficoll and collagen/Ficoll/genipin films showed the highest WI38 fibroblast attachment than any other scaffold (p < 0.003) and significantly greater WI38 fibroblast metabolic activity than other scaffolds (p < 0.001). By day 6. collagen/Ficoll/genipin films also induced higher and more aligned fibronectin matrix deposition than other scaffolds. Overall, this study indicates the suitability of collagen/Ficoll/genipin for tissue engineering applications. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/12211 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Satyam, A., Subramanian, G. S., Raghunath, M., Pandit, A., & Zeugolis, D. I. (2012). In vitro evaluation of Ficoll‐enriched and genipin‐stabilised collagen scaffolds. Journal of Tissue Engineering and Regenerative Medicine, 8(3), 233–241. https://doi.org/10.1002/term.1522
Satyam, A. et al. (2012) ‘In vitro evaluation of Ficoll‐enriched and genipin‐stabilised collagen scaffolds’, Journal of Tissue Engineering and Regenerative Medicine, 8(3), pp. 233–241. Available at: https://doi.org/10.1002/term.1522.
A. Satyam, G. S. Subramanian, M. Raghunath, A. Pandit, and D. I. Zeugolis, “In vitro evaluation of Ficoll‐enriched and genipin‐stabilised collagen scaffolds,” Journal of Tissue Engineering and Regenerative Medicine, vol. 8, no. 3, pp. 233–241, May 2012, doi: 10.1002/term.1522.
SATYAM, A., G. S. SUBRAMANIAN, M. RAGHUNATH, A. PANDIT und D. I. ZEUGOLIS, 2012. In vitro evaluation of Ficoll‐enriched and genipin‐stabilised collagen scaffolds. Journal of Tissue Engineering and Regenerative Medicine. Mai 2012. Bd. 8, Nr. 3, S. 233–241. DOI 10.1002/term.1522
Satyam, A., G. S. Subramanian, M. Raghunath, A. Pandit, and D. I. Zeugolis. 2012. “In Vitro Evaluation of Ficoll‐Enriched and Genipin‐Stabilised Collagen Scaffolds.” Journal of Tissue Engineering and Regenerative Medicine 8 (3): 233–41. https://doi.org/10.1002/term.1522.
Satyam, A., et al. “In Vitro Evaluation of Ficoll‐Enriched and Genipin‐Stabilised Collagen Scaffolds.” Journal of Tissue Engineering and Regenerative Medicine, vol. 8, no. 3, May 2012, pp. 233–41, https://doi.org/10.1002/term.1522.
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