Title: In vitro evaluation of Ficoll‐enriched and genipin‐stabilised collagen scaffolds
Authors : Satyam, A.
Subramanian, G. S.
Raghunath, M.
Pandit, A.
Zeugolis, D. I.
Published in : Journal of tissue engineering and regenerative medicine
Volume(Issue) : 8
Issue : 3
Pages : 233
Pages to: 241
Publisher / Ed. Institution : Wiley
Issue Date: May-2012
License (according to publishing contract) : Licence according to publishing contract
Type of review: Peer review (Publication)
Language : English
Subject (DDC) : 572: Biochemistry
610: Medicine and health
Abstract: Polysaccharides are frequently incorporated into scaffolds for tissue engineering applications to improve mechanical and biological properties. We evaluated the influence of a Ficoll® scaffold on collagen films, a scaffold that is extensively used for soft and hard tissue repair. To avoid cytotoxicity issues associated with chemical reagents, the influence of genipin, a naturally occurring crosslinking agent, was assessed. Ultra‐structural level collagen films formed with and without Ficoll showed a fine fibrillar structure whereas genipin crosslinked films showed a coarse fibrillar and partially nodular structure. In contrast, glutaraldehyde crosslinked films lost their fibrillar pattern. Crosslinking significantly increased denaturation temperature (p < 0.001), stress (p < 0.0001) and force (p < 0.0001) at break. Collagen/Ficoll and collagen/Ficoll/genipin films showed the highest WI38 fibroblast attachment than any other scaffold (p < 0.003) and significantly greater WI38 fibroblast metabolic activity than other scaffolds (p < 0.001). By day 6. collagen/Ficoll/genipin films also induced higher and more aligned fibronectin matrix deposition than other scaffolds. Overall, this study indicates the suitability of collagen/Ficoll/genipin for tissue engineering applications.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific Journal
DOI : 10.1002/term.1522
ISSN: 1932-7005
URI: https://digitalcollection.zhaw.ch/handle/11475/12211
Appears in Collections:Publikationen Life Sciences und Facility Management

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