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https://doi.org/10.21256/zhaw-4751| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Effects of macromolecular crowding on human adipose stem cell culture in fetal bovine serum, human serum and defined xeno-free/serum-free conditions |
| Autor/-in: | Patrikoski, Mimmi Hui Ching Lee, Michelle Mäkinen, Laura Ang, Xiu Min Mannerström, Bettina Raghunath, Michael Miettinen, Susanna |
| DOI: | 10.21256/zhaw-4751 10.1155/2017/6909163 |
| Erschienen in: | Stem Cells International |
| Band(Heft): | 2017 |
| Heft: | 6909163 |
| Erscheinungsdatum: | 2017 |
| Verlag / Hrsg. Institution: | Hindawi |
| ISSN: | 1687-9678 1687-966X |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 571: Physiologie und verwandte Themen 616: Innere Medizin und Krankheiten |
| Zusammenfassung: | Microenvironment plays an important role for stem cell proliferation and differentiation. Macromolecular crowding (MMC) was recently shown to assist stem cells in forming their own matrix microenvironment in vitro. The ability of MMC to support adipose stem cell (ASC) proliferation, metabolism, and multilineage differentiation was studied under different conditions: fetal bovine serum- (FBS-) and human serum- (HS-) based media and xeno- and serum-free (XF/SF) media. Furthermore, the immunophenotype of ASCs under MMC was evaluated. The proliferative capacity of ASCs under MMC was attenuated in each condition. However, osteogenic differentiation was enhanced under MMC, shown by increased deposition of mineralized matrix in FBS and HS cultures. Likewise, significantly greater lipid droplet accumulation and increased collagen IV deposition indicated enhanced adipogenesis under MMC in FBS and HS cultures. In contrast, chondrogenic differentiation was attenuated in ASCs expanded under MMC. The ASC immunophenotype was maintained under MMC with significantly higher expression of CD54. However, MMC impaired metabolic activity and differentiation capacity of ASCs in XF/SF conditions. Both the supportive and inhibitory effects of MMC on ASC are culture condition dependent. In the presence of serum, MMC maintains ASC immunophenotype and enhances adipogenic and osteogenic differentiation at the cost of reduced proliferation. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/12188 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | CC BY 4.0: Namensnennung 4.0 International |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
Dateien zu dieser Ressource:
| Datei | Beschreibung | Größe | Format | |
|---|---|---|---|---|
| 2017_Patrikoski_et_al_Effects_of_macromolecular_crowding_on_human_adipose_stem_cell_culture.pdf | 8.14 MB | Adobe PDF |  Öffnen/Anzeigen |
Zur Langanzeige
Patrikoski, M., Hui Ching Lee, M., Mäkinen, L., Ang, X. M., Mannerström, B., Raghunath, M., & Miettinen, S. (2017). Effects of macromolecular crowding on human adipose stem cell culture in fetal bovine serum, human serum and defined xeno-free/serum-free conditions. Stem Cells International, 2017(6909163). https://doi.org/10.21256/zhaw-4751
Patrikoski, M. et al. (2017) ‘Effects of macromolecular crowding on human adipose stem cell culture in fetal bovine serum, human serum and defined xeno-free/serum-free conditions’, Stem Cells International, 2017(6909163). Available at: https://doi.org/10.21256/zhaw-4751.
M. Patrikoski et al., “Effects of macromolecular crowding on human adipose stem cell culture in fetal bovine serum, human serum and defined xeno-free/serum-free conditions,” Stem Cells International, vol. 2017, no. 6909163, 2017, doi: 10.21256/zhaw-4751.
PATRIKOSKI, Mimmi, Michelle HUI CHING LEE, Laura MÄKINEN, Xiu Min ANG, Bettina MANNERSTRÖM, Michael RAGHUNATH und Susanna MIETTINEN, 2017. Effects of macromolecular crowding on human adipose stem cell culture in fetal bovine serum, human serum and defined xeno-free/serum-free conditions. Stem Cells International. 2017. Bd. 2017, Nr. 6909163. DOI 10.21256/zhaw-4751
Patrikoski, Mimmi, Michelle Hui Ching Lee, Laura Mäkinen, Xiu Min Ang, Bettina Mannerström, Michael Raghunath, and Susanna Miettinen. 2017. “Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum and Defined Xeno-Free/Serum-Free Conditions.” Stem Cells International 2017 (6909163). https://doi.org/10.21256/zhaw-4751.
Patrikoski, Mimmi, et al. “Effects of Macromolecular Crowding on Human Adipose Stem Cell Culture in Fetal Bovine Serum, Human Serum and Defined Xeno-Free/Serum-Free Conditions.” Stem Cells International, vol. 2017, no. 6909163, 2017, https://doi.org/10.21256/zhaw-4751.
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