Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-4751
Title: Effects of macromolecular crowding on human adipose stem cell culture in fetal bovine serum, human serum and defined xeno-free/serum-free conditions
Authors : Patrikoski, Mimmi
Hui Ching Lee, Michelle
Mäkinen, Laura
Ang, Xiu Min
Mannerström, Bettina
Raghunath, Michael
Miettinen, Susanna
Published in : Stem cells international
Volume(Issue) : 2017
Issue : 6909163
Publisher / Ed. Institution : Hindawi
Issue Date: 2017
License (according to publishing contract) : CC BY 4.0: Attribution 4.0 International
Type of review: Peer review (Publication)
Language : English
Subject (DDC) : 571: Physiology and related subjects
616: Internal medicine and diseases
Abstract: Microenvironment plays an important role for stem cell proliferation and differentiation. Macromolecular crowding (MMC) was recently shown to assist stem cells in forming their own matrix microenvironment in vitro. The ability of MMC to support adipose stem cell (ASC) proliferation, metabolism, and multilineage differentiation was studied under different conditions: fetal bovine serum- (FBS-) and human serum- (HS-) based media and xeno- and serum-free (XF/SF) media. Furthermore, the immunophenotype of ASCs under MMC was evaluated. The proliferative capacity of ASCs under MMC was attenuated in each condition. However, osteogenic differentiation was enhanced under MMC, shown by increased deposition of mineralized matrix in FBS and HS cultures. Likewise, significantly greater lipid droplet accumulation and increased collagen IV deposition indicated enhanced adipogenesis under MMC in FBS and HS cultures. In contrast, chondrogenic differentiation was attenuated in ASCs expanded under MMC. The ASC immunophenotype was maintained under MMC with significantly higher expression of CD54. However, MMC impaired metabolic activity and differentiation capacity of ASCs in XF/SF conditions. Both the supportive and inhibitory effects of MMC on ASC are culture condition dependent. In the presence of serum, MMC maintains ASC immunophenotype and enhances adipogenic and osteogenic differentiation at the cost of reduced proliferation.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Publication type: Article in scientific Journal
DOI : 10.1155/2017/6909163
10.21256/zhaw-4751
ISSN: 1687-9678
1687-966X
URI: https://digitalcollection.zhaw.ch/handle/11475/12188
Appears in Collections:Publikationen Life Sciences und Facility Management



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