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https://doi.org/10.21256/zhaw-4818| Publikationstyp: | Weiteres (textuelles Material) |
| Titel: | Anoxygenic photosynthesis and dark carbon metabolism under micro-oxic conditions in the purple sulfur bacterium "Thiodictyon syntrophicum" nov. strain Cad16T |
| Autor/-in: | Luedin, Samuel M. Storelli, Nicola Danza, Francesco Roman, Samuele Wittwer, Matthias Pothier, Joël Tonolla, Mauro |
| DOI: | 10.21256/zhaw-4818 10.1101/420927 |
| Erscheinungsdatum: | 18-Sep-2018 |
| Verlag / Hrsg. Institution: | bioRxiv |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 570: Biologie |
| Zusammenfassung: | The microbial ecosystem of the meromictic Lake Cadagno (Ticino, Swiss Alps) has been studied intensively to understand metabolic functions driven by the highly abundant anoxygenic phototrophic sulfur bacteria of the families Chromatiaceae and Chlorobiaceae. It was found that the sequenced isolate "Thiodictyon syntrophicum" nov. sp. str. Cad16T, belonging to the Chromatiaceae, may fix 26% of all bulk inorganic carbon in the chemocline at day and night. With this study, we elucidated the mode of dark carbon fixation of str. Cad16T with a combination of long-term monitoring of key physicochemical parameters with CTD, 14C-incorporation experiments and quantitative proteomics of in situ dialysis bag incubations of pure cultures. Regular vertical CTD profiling during the study period in summer 2017 revealed that the chemocline sank from 12 to 14 m which was accompanied by a bloom of cyanobacteria and the subsequent oxygenation of the deeper water column. Sampling was performed both day and night in September. While CO2 assimilation rates were higher during the light period, the relative change in the proteome (663 quantified proteins) was only 1% of all CDS encoded in str. Cad16T. Oxidative respiration was thereby upregulated at light, whereas stress-related mechanisms prevailed during the night. These results indicate that the low light availability due to high cell concentrations and the oxygenation of the chemocline induced a mixotrophic growth in str. Cad16T. The complete proteome data have been deposited to the ProteomeXchange with identifier PXD010641. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/10946 |
| Volltext Version: | Entwurf |
| Lizenz (gemäss Verlagsvertrag): | CC BY-NC 4.0: Namensnennung - Nicht kommerziell 4.0 International |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Umwelt und Natürliche Ressourcen (IUNR) |
| Publiziert im Rahmen des ZHAW-Projekts: | High-throughput proteomics of Thiodictyon synthrophicum strain Cad16T |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
Dateien zu dieser Ressource:
| Datei | Beschreibung | Größe | Format | |
|---|---|---|---|---|
| 2018_Luedin_Anoxygenic_photosynthesis_and_dark_carbon_metabolism.pdf | 2.01 MB | Adobe PDF |  Öffnen/Anzeigen |
Zur Langanzeige
Luedin, S. M., Storelli, N., Danza, F., Roman, S., Wittwer, M., Pothier, J., & Tonolla, M. (2018). Anoxygenic photosynthesis and dark carbon metabolism under micro-oxic conditions in the purple sulfur bacterium “Thiodictyon syntrophicum” nov. strain Cad16T. bioRxiv. https://doi.org/10.21256/zhaw-4818
Luedin, S.M. et al. (2018) Anoxygenic photosynthesis and dark carbon metabolism under micro-oxic conditions in the purple sulfur bacterium ‘Thiodictyon syntrophicum’ nov. strain Cad16T. bioRxiv. Available at: https://doi.org/10.21256/zhaw-4818.
S. M. Luedin et al., Anoxygenic photosynthesis and dark carbon metabolism under micro-oxic conditions in the purple sulfur bacterium “Thiodictyon syntrophicum” nov. strain Cad16T. bioRxiv, 2018. doi: 10.21256/zhaw-4818.
LUEDIN, Samuel M., Nicola STORELLI, Francesco DANZA, Samuele ROMAN, Matthias WITTWER, Joël POTHIER und Mauro TONOLLA, 2018. Anoxygenic photosynthesis and dark carbon metabolism under micro-oxic conditions in the purple sulfur bacterium „Thiodictyon syntrophicum“ nov. strain Cad16T. bioRxiv
Luedin, Samuel M., Nicola Storelli, Francesco Danza, Samuele Roman, Matthias Wittwer, Joël Pothier, and Mauro Tonolla. 2018. Anoxygenic Photosynthesis and Dark Carbon Metabolism under Micro-Oxic Conditions in the Purple Sulfur Bacterium “Thiodictyon Syntrophicum” Nov. Strain Cad16T. bioRxiv. https://doi.org/10.21256/zhaw-4818.
Luedin, Samuel M., et al. Anoxygenic Photosynthesis and Dark Carbon Metabolism under Micro-Oxic Conditions in the Purple Sulfur Bacterium “Thiodictyon Syntrophicum” Nov. Strain Cad16T. bioRxiv, 2018, https://doi.org/10.21256/zhaw-4818.
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