Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-4818
Title: Anoxygenic photosynthesis and dark carbon metabolism under micro-oxic conditions in the purple sulfur bacterium "Thiodictyon syntrophicum" nov. strain Cad16T
Authors : Luedin, Samuel M.
Storelli, Nicola
Danza, Francesco
Roman, Samuele
Wittwer, Matthias
Pothier, Joël
Tonolla, Mauro
Publisher / Ed. Institution : Cold Spring Harbor Laboratory
Publisher / Ed. Institution: bioRxiv
Issue Date: 18-Sep-2018
License (according to publishing contract) : CC BY-NC 4.0: Attribution - Non commercial 4.0 International
Language : English
Subject (DDC) : 570: Biology
Abstract: The microbial ecosystem of the meromictic Lake Cadagno (Ticino, Swiss Alps) has been studied intensively to understand metabolic functions driven by the highly abundant anoxygenic phototrophic sulfur bacteria of the families Chromatiaceae and Chlorobiaceae. It was found that the sequenced isolate "Thiodictyon syntrophicum" nov. sp. str. Cad16T, belonging to the Chromatiaceae, may fix 26% of all bulk inorganic carbon in the chemocline at day and night. With this study, we elucidated the mode of dark carbon fixation of str. Cad16T with a combination of long-term monitoring of key physicochemical parameters with CTD, 14C-incorporation experiments and quantitative proteomics of in situ dialysis bag incubations of pure cultures. Regular vertical CTD profiling during the study period in summer 2017 revealed that the chemocline sank from 12 to 14 m which was accompanied by a bloom of cyanobacteria and the subsequent oxygenation of the deeper water column. Sampling was performed both day and night in September. While CO2 assimilation rates were higher during the light period, the relative change in the proteome (663 quantified proteins) was only 1% of all CDS encoded in str. Cad16T. Oxidative respiration was thereby upregulated at light, whereas stress-related mechanisms prevailed during the night. These results indicate that the low light availability due to high cell concentrations and the oxygenation of the chemocline induced a mixotrophic growth in str. Cad16T. The complete proteome data have been deposited to the ProteomeXchange with identifier PXD010641.
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Natural Resource Sciences (IUNR)
Publication type: Other (textual)
DOI : 10.1101/420927
10.21256/zhaw-4818
URI: https://digitalcollection.zhaw.ch/handle/11475/10946
Published as part of the ZHAW project : High-throughput proteomics of Thiodictyon synthrophicum strain Cad16T
Appears in Collections:Publikationen Life Sciences und Facility Management

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