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https://doi.org/10.21256/zhaw-4807| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Cultivation strategies to enhance productivity of Pichia pastoris : a review |
| Autor/-in: | Looser, Verena Brühlmann, Béla Bumbak, Fabian Stenger, Chantal Costa, Merixtell Camattari, Andrea Fotiadis, Dimitrios Kovar, Karin |
| DOI: | 10.21256/zhaw-4807 10.1016/j.biotechadv.2015.05.008 |
| Erschienen in: | Biotechnology Advances |
| Band(Heft): | 33 |
| Seite(n): | 1177 |
| Seiten bis: | 1193 |
| Erscheinungsdatum: | 25-Mai-2015 |
| Verlag / Hrsg. Institution: | Elsevier |
| ISSN: | 0734-9750 1873-1899 |
| Sprache: | Englisch |
| Schlagwörter: | Pichia pastoris; Recombinant protein; Production process development; Fermentation; Productivity |
| Fachgebiet (DDC): | 570: Biologie |
| Zusammenfassung: | Pichia pastoris, a methylotrophic yeast, is an established system for the production of heterologous proteins, particularly biopharmaceuticals and industrial enzymes. To maximise and optimise the production of recombinant products, recent molecular research has focused on numerous issues including the design of expression vectors, optimisation of gene copy number, co-expression of secretory proteins such as chaperones, engineering of glycosylation and secretory pathways, etc. However, the physiological effects of different cultivation strategies are often difficult to separate from the molecular effects of the gene construct (e.g., cellular stress through over-expression or incorrect post-translational processing). Hence, overall system optimisation is difficult, even though it is urgently required in order to describe and understand the behaviour of new molecular constructs. This review focuses on particular aspects of recombinant protein production related to variations in biomass growth and their implications for strain design and screening, as well as on the concept of rational comparisons between cultivation systems for the development of specific production processes in bioreactors. The relationship between specific formation rates of secreted recombinant proteins, qp, and specific growth rates, μ, has been analysed in a conceptual attempt to compare different systems, particularly those based on AOX1/methanol and GAP/glucose, and this has now evolved into a pivotal concept for bioprocess engineering of P. pastoris. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/10880 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | CC BY-NC-ND 4.0: Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
Dateien zu dieser Ressource:
| Datei | Beschreibung | Größe | Format | |
|---|---|---|---|---|
| 2015_Looser_Cultivation_Strategies_To_Enhance.pdf | 1.36 MB | Adobe PDF |  Öffnen/Anzeigen |
Zur Langanzeige
Looser, V., Brühlmann, B., Bumbak, F., Stenger, C., Costa, M., Camattari, A., Fotiadis, D., & Kovar, K. (2015). Cultivation strategies to enhance productivity of Pichia pastoris : a review. Biotechnology Advances, 33, 1177–1193. https://doi.org/10.21256/zhaw-4807
Looser, V. et al. (2015) ‘Cultivation strategies to enhance productivity of Pichia pastoris : a review’, Biotechnology Advances, 33, pp. 1177–1193. Available at: https://doi.org/10.21256/zhaw-4807.
V. Looser et al., “Cultivation strategies to enhance productivity of Pichia pastoris : a review,” Biotechnology Advances, vol. 33, pp. 1177–1193, May 2015, doi: 10.21256/zhaw-4807.
LOOSER, Verena, Béla BRÜHLMANN, Fabian BUMBAK, Chantal STENGER, Merixtell COSTA, Andrea CAMATTARI, Dimitrios FOTIADIS und Karin KOVAR, 2015. Cultivation strategies to enhance productivity of Pichia pastoris : a review. Biotechnology Advances. 25 Mai 2015. Bd. 33, S. 1177–1193. DOI 10.21256/zhaw-4807
Looser, Verena, Béla Brühlmann, Fabian Bumbak, Chantal Stenger, Merixtell Costa, Andrea Camattari, Dimitrios Fotiadis, and Karin Kovar. 2015. “Cultivation Strategies to Enhance Productivity of Pichia Pastoris : A Review.” Biotechnology Advances 33 (May): 1177–93. https://doi.org/10.21256/zhaw-4807.
Looser, Verena, et al. “Cultivation Strategies to Enhance Productivity of Pichia Pastoris : A Review.” Biotechnology Advances, vol. 33, May 2015, pp. 1177–93, https://doi.org/10.21256/zhaw-4807.
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