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Publication type: Article in scientific journal
Type of review: Peer review (publication)
Title: Cultivation strategies to enhance productivity of Pichia pastoris : a review
Authors: Looser, Verena
Brühlmann, Béla
Bumbak, Fabian
Stenger, Chantal
Costa, Merixtell
Camattari, Andrea
Fotiadis, Dimitrios
Kovar, Karin
DOI: 10.21256/zhaw-4807
Published in: Biotechnology Advances
Volume(Issue): 33
Pages: 1177
Pages to: 1193
Issue Date: 25-May-2015
Publisher / Ed. Institution: Elsevier
ISSN: 0734-9750
Language: English
Subjects: Pichia pastoris; Recombinant protein; Production process development; Fermentation; Productivity
Subject (DDC): 570: Biology
Abstract: Pichia pastoris, a methylotrophic yeast, is an established system for the production of heterologous proteins, particularly biopharmaceuticals and industrial enzymes. To maximise and optimise the production of recombinant products, recent molecular research has focused on numerous issues including the design of expression vectors, optimisation of gene copy number, co-expression of secretory proteins such as chaperones, engineering of glycosylation and secretory pathways, etc. However, the physiological effects of different cultivation strategies are often difficult to separate from the molecular effects of the gene construct (e.g., cellular stress through over-expression or incorrect post-translational processing). Hence, overall system optimisation is difficult, even though it is urgently required in order to describe and understand the behaviour of new molecular constructs. This review focuses on particular aspects of recombinant protein production related to variations in biomass growth and their implications for strain design and screening, as well as on the concept of rational comparisons between cultivation systems for the development of specific production processes in bioreactors. The relationship between specific formation rates of secreted recombinant proteins, qp, and specific growth rates, μ, has been analysed in a conceptual attempt to compare different systems, particularly those based on AOX1/methanol and GAP/glucose, and this has now evolved into a pivotal concept for bioprocess engineering of P. pastoris.
Fulltext version: Published version
License (according to publishing contract): CC BY-NC-ND 4.0: Attribution - Non commercial - No derivatives 4.0 International
Departement: Life Sciences and Facility Management
Organisational Unit: Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:Publikationen Life Sciences und Facility Management

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