| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5-bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes |
| Autor/-in: | Crottet, Pascal Meyer, Daniel M. Rohrer, Jack Spiess, Martin |
| DOI: | 10.1091/mbc.e02-05-0309 |
| Erschienen in: | Molecular Biology of the Cell |
| Band(Heft): | 13 |
| Heft: | 10 |
| Seite(n): | 3672 |
| Seiten bis: | 3682 |
| Erscheinungsdatum: | Okt-2002 |
| Verlag / Hrsg. Institution: | American Society for Cell Biology |
| ISSN: | 1939-4586 1059-1524 |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 571: Physiologie und verwandte Themen 572: Biochemie |
| Zusammenfassung: | At the trans-Golgi network, clathrin coats containing AP-1 adaptor complexes are formed in an ARF1-dependent manner, generating vesicles transporting cargo proteins to endosomes. The mechanism of site-specific targeting of AP-1 and the role of cargo are poorly understood. We have developed an in vitro assay to study the recruitment of purified AP-1 adaptors to chemically defined liposomes presenting peptides corresponding to tyrosine-based sorting motifs. AP-1 recruitment was found to be dependent on myristoylated ARF1, GTP or nonhydrolyzable GTP-analogs, tyrosine signals, and small amounts of phosphoinositides, most prominently phosphatidylinositol 4,5-bisphosphate, in the absence of any additional cytosolic or membrane bound proteins. AP-1 from cytosol could be recruited to a tyrosine signal independently of the lipid composition, but the rate of recruitment was increased by phosphatidylinositol 4,5-bisphosphate. The results thus indicate that cargo proteins are involved in coat recruitment and that the local lipid composition contributes to specifying the site of vesicle formation. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/6813 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Crottet, P., Meyer, D. M., Rohrer, J., & Spiess, M. (2002). ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5-bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes. Molecular Biology of the Cell, 13(10), 3672–3682. https://doi.org/10.1091/mbc.e02-05-0309
Crottet, P. et al. (2002) ‘ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5-bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes’, Molecular Biology of the Cell, 13(10), pp. 3672–3682. Available at: https://doi.org/10.1091/mbc.e02-05-0309.
P. Crottet, D. M. Meyer, J. Rohrer, and M. Spiess, “ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5-bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes,” Molecular Biology of the Cell, vol. 13, no. 10, pp. 3672–3682, Oct. 2002, doi: 10.1091/mbc.e02-05-0309.
CROTTET, Pascal, Daniel M. MEYER, Jack ROHRER und Martin SPIESS, 2002. ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5-bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes. Molecular Biology of the Cell. Oktober 2002. Bd. 13, Nr. 10, S. 3672–3682. DOI 10.1091/mbc.e02-05-0309
Crottet, Pascal, Daniel M. Meyer, Jack Rohrer, and Martin Spiess. 2002. “ARF1.GTP, Tyrosine-Based Signals, and Phosphatidylinositol 4,5-Bisphosphate Constitute a Minimal Machinery to Recruit the AP-1 Clathrin Adaptor to Membranes.” Molecular Biology of the Cell 13 (10): 3672–82. https://doi.org/10.1091/mbc.e02-05-0309.
Crottet, Pascal, et al. “ARF1.GTP, Tyrosine-Based Signals, and Phosphatidylinositol 4,5-Bisphosphate Constitute a Minimal Machinery to Recruit the AP-1 Clathrin Adaptor to Membranes.” Molecular Biology of the Cell, vol. 13, no. 10, Oct. 2002, pp. 3672–82, https://doi.org/10.1091/mbc.e02-05-0309.
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