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https://doi.org/10.21256/zhaw-4128| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | In vitro culture development and polyphenolics production of Artemisia alba Turra |
| Authors: | Koleva, Petya Wolfram, Evelyn Pedrussio, Simona Raynova, Yuliana Evstatieva, Luba Danova, Kalina |
| DOI: | 10.21256/zhaw-4128 |
| Published in: | Journal of Biosciences and Biotechnology |
| Page(s): | 131 |
| Pages to: | 136 |
| Issue Date: | Oct-2015 |
| Publisher / Ed. Institution: | Plovdiv University Press Paisii Hilendarski |
| ISSN: | 1314-6238 |
| Language: | English |
| Subjects: | Morphogenesis; In vitro culture; Polyphenols; Artemisia alba |
| Subject (DDC): | 615: Pharmacology and therapeutics |
| Abstract: | Artemisia alba Turra is an aromatic plant, characterized by a high variability of the terpenoid profile of its essential oil. In previous research, in vitro shoots of the plant were developed, aiming at elucidation of the effects of plant growth regulators on essential oil production. Though less information is available in literature regarding the non-volatile components of the plant, a number of works report on the presence of compounds with coumarin, flavonoid and sesquiterpene structure which might attribute to the pharmacological activity of the plant. In the present work, different lines of differentiated and non-differentiated in vitro cultures of the plant have been developed in solid and liquid media. The potential of these lines to produce compounds with phenolic and flavonoid structure has been studied. In differentiated shoot cultures, low benzyl adenine (BA) concentration alone or in combination with different indole-3-butyric acid (IBA) concentrations increased the polyphenolic levels as compared with plant growth regulators free control, as well with media with high BA alone or combined with IBA. The content of these compounds was also low when IBA was applied alone. In non-differentiated cell aggregate cultures, 1-naphthaleneacetic acid (NAA) in combination with BA significantly increased polyphenolics as compared with IBA. Observations on the morphology of the aggregates formed in the two media suggested that the more compact structure and larger size of aggregates as a result of NAA supplementation might be decisive for the higher polyphenolics productivity, as compared with IBA. |
| Further description: | Open Access Journal Second National Youth Conference “Biological sciences for a better future”, Plovdiv, October 30-31, 2015. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/3011 |
| Fulltext version: | Published version |
| License (according to publishing contract): | CC BY-NC-ND 3.0: Attribution - Non commercial - No derivatives 3.0 Unported |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
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|---|---|---|---|---|
| 2015_Koleva_In vitro culture development_Journal of BioSciences and Biotechnology.pdf | 587.67 kB | Adobe PDF |  View/Open |
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Koleva, P., Wolfram, E., Pedrussio, S., Raynova, Y., Evstatieva, L., & Danova, K. (2015). In vitro culture development and polyphenolics production of Artemisia alba Turra. Journal of Biosciences and Biotechnology, 131–136. https://doi.org/10.21256/zhaw-4128
Koleva, P. et al. (2015) ‘In vitro culture development and polyphenolics production of Artemisia alba Turra’, Journal of Biosciences and Biotechnology, pp. 131–136. Available at: https://doi.org/10.21256/zhaw-4128.
P. Koleva, E. Wolfram, S. Pedrussio, Y. Raynova, L. Evstatieva, and K. Danova, “In vitro culture development and polyphenolics production of Artemisia alba Turra,” Journal of Biosciences and Biotechnology, pp. 131–136, Oct. 2015, doi: 10.21256/zhaw-4128.
KOLEVA, Petya, Evelyn WOLFRAM, Simona PEDRUSSIO, Yuliana RAYNOVA, Luba EVSTATIEVA und Kalina DANOVA, 2015. In vitro culture development and polyphenolics production of Artemisia alba Turra. Journal of Biosciences and Biotechnology. Oktober 2015. S. 131–136. DOI 10.21256/zhaw-4128
Koleva, Petya, Evelyn Wolfram, Simona Pedrussio, Yuliana Raynova, Luba Evstatieva, and Kalina Danova. 2015. “In Vitro Culture Development and Polyphenolics Production of Artemisia Alba Turra.” Journal of Biosciences and Biotechnology, October, 131–36. https://doi.org/10.21256/zhaw-4128.
Koleva, Petya, et al. “In Vitro Culture Development and Polyphenolics Production of Artemisia Alba Turra.” Journal of Biosciences and Biotechnology, Oct. 2015, pp. 131–36, https://doi.org/10.21256/zhaw-4128.
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