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https://doi.org/10.21256/zhaw-27156| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | Cultivation of the PHB-producing cyanobacterium Synechococcus leopoliensis in a pilot-scale open system using nitrogen from waste streams |
| Authors: | Mariotto, Marina Egloff, Sophia Fritz, Ines Refardt, Dominik |
| et. al: | No |
| DOI: | 10.1016/j.algal.2023.103013 10.21256/zhaw-27156 |
| Published in: | Algal Research |
| Volume(Issue): | 70 |
| Issue: | 103013 |
| Issue Date: | 2023 |
| Publisher / Ed. Institution: | Elsevier |
| ISSN: | 2211-9264 |
| Language: | English |
| Subjects: | Wastewater treatment; Liquid digestate; Aquaculture water; Bioplastic; Biopolymer; Nitrogen mass balance |
| Subject (DDC): | 572: Biochemistry |
| Abstract: | PHB-producing cyanobacteria may provide the raw material for bio-based and biodegradable plastics. To commercialize photoautotrophic PHB production, their cultivation needs to be scaled up in open systems and to reduce costs and increase sustainability, nutrients must be obtained from waste streams. Here, the feasibility of these steps was verified. Different PHB-producing cyanobacteria were compared in laboratory-scale cultivations using either water from recirculating aquaculture systems or pre-processed liquid digestate as nutrient sources. Then, Synechococcus leopoliensis was cultivated in an open thin-layer photobioreactor (18 m2, 200 L), were growth in mineral Z-medium was again compared to said waste streams. All cultivations were successful. Cultivation in mineral medium resulted in both the highest final biomass yield (6 g L−1) and productivity (0.7 g L−1 d−1). Both waste stream-based media showed lower biomass yields and productivities (2 g L−1 and 0.25–0.3 g L−1 d−1). However, due to differences in the cultivation conditions (e.g., temperature, nutrient supply), final biomass yield and productivity do not represent the performance of the cultivations adequately. Relative parameters such as nitrogen and energy conversion ratios indicate that cultivation with aquaculture water suffered from insufficient nitrogen supply to the culture, whereas the use pre-processed liquid digestate resulted in a substantial loss of nitrogen due to volatilization. All cultivations in the open system were continued in the laboratory, where cultures were starved for ten days under nutrient-depleted conditions (without nitrogen, phosphorus, or both). While PHB accumulation occurred, concentrations were comparatively low (< 1%dw). The comparison of the results suggests that PHB yields were influenced more by the initial cultivation condition than by the specific type of nutrient depletion. Thus, while the cultivation with waste streams in an open system is feasible, environmental parameters seem to influence PHB yields considerably and must be considered for the optimization of the complete process. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/27156 |
| Related research data: | https://doi.org/10.5061/dryad.0k6djhb33 |
| Fulltext version: | Published version |
| License (according to publishing contract): | CC BY 4.0: Attribution 4.0 International |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Natural Resource Sciences (IUNR) |
| Published as part of the ZHAW project: | BIOMAT (Integrated Bio-based Materials Value Chains) |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 2023_Mariotto-etal_Synechoccus-leopoliensis-cultivation_AlgalResearch.pdf | Article | 1.08 MB | Adobe PDF |  View/Open |
| 2023_Mariotto-etal_Synechoccus-leopoliensis-cultivation_AlgalResearch_Suppl.pdf | Supplementary Material | 1.13 MB | Adobe PDF |  View/Open |
Show full item record
Mariotto, M., Egloff, S., Fritz, I., & Refardt, D. (2023). Cultivation of the PHB-producing cyanobacterium Synechococcus leopoliensis in a pilot-scale open system using nitrogen from waste streams. Algal Research, 70(103013). https://doi.org/10.1016/j.algal.2023.103013
Mariotto, M. et al. (2023) ‘Cultivation of the PHB-producing cyanobacterium Synechococcus leopoliensis in a pilot-scale open system using nitrogen from waste streams’, Algal Research, 70(103013). Available at: https://doi.org/10.1016/j.algal.2023.103013.
M. Mariotto, S. Egloff, I. Fritz, and D. Refardt, “Cultivation of the PHB-producing cyanobacterium Synechococcus leopoliensis in a pilot-scale open system using nitrogen from waste streams,” Algal Research, vol. 70, no. 103013, 2023, doi: 10.1016/j.algal.2023.103013.
MARIOTTO, Marina, Sophia EGLOFF, Ines FRITZ und Dominik REFARDT, 2023. Cultivation of the PHB-producing cyanobacterium Synechococcus leopoliensis in a pilot-scale open system using nitrogen from waste streams. Algal Research. 2023. Bd. 70, Nr. 103013. DOI 10.1016/j.algal.2023.103013
Mariotto, Marina, Sophia Egloff, Ines Fritz, and Dominik Refardt. 2023. “Cultivation of the PHB-Producing Cyanobacterium Synechococcus Leopoliensis in a Pilot-Scale Open System Using Nitrogen from Waste Streams.” Algal Research 70 (103013). https://doi.org/10.1016/j.algal.2023.103013.
Mariotto, Marina, et al. “Cultivation of the PHB-Producing Cyanobacterium Synechococcus Leopoliensis in a Pilot-Scale Open System Using Nitrogen from Waste Streams.” Algal Research, vol. 70, no. 103013, 2023, https://doi.org/10.1016/j.algal.2023.103013.
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