Towards detecting genotoxic chemicals in food packaging at thresholds of toxicological concern using bioassays with high-performance thin-layer chromatography

Bergmann, Alan J.; Breitenbach, Milena; Muñoz, Celine; Simon, Eszter; McCombie, Gregor; Biedermann, Maurus; Schönborn, Andreas; Vermeirssen, Etiënne L.M.

doi:10.1016/j.fpsl.2023.101052

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-27078

Full metadata record

DC Field	Value	Language
dc.contributor.author	Bergmann, Alan J.	-
dc.contributor.author	Breitenbach, Milena	-
dc.contributor.author	Muñoz, Celine	-
dc.contributor.author	Simon, Eszter	-
dc.contributor.author	McCombie, Gregor	-
dc.contributor.author	Biedermann, Maurus	-
dc.contributor.author	Schönborn, Andreas	-
dc.contributor.author	Vermeirssen, Etiënne L.M.	-
dc.date.accessioned	2023-02-17T10:37:19Z	-
dc.date.available	2023-02-17T10:37:19Z	-
dc.date.issued	2023-04	-
dc.identifier.issn	2214-2894	de_CH
dc.identifier.uri	https://digitalcollection.zhaw.ch/handle/11475/27078	-
dc.description.abstract	High-performance thin-layer chromatography (HPTLC)-bioassays are promising new methods for detecting bioactive chemicals in food packaging. Here, we test whether direct-acting genotoxic chemicals are detectable in food contact materials (FCM) using HPTLC-bioassays. First, an interactive worksheet lays out steps to calculate needed detection limits in (bio)analytical methods from regulatory limits, including thresholds of toxicological concern (TTC). Second, we show that the sensitivity of a HPTLC-genotoxicity assay to low doses of chemicals, including food contact chemicals, is greater than a standardized microtiter plate version and in vitro assays already reported. Third, using HPTLC, we detected genotoxicity in extracts of FCM, and not in simulated migrates of FCM. Applying the worksheet to calculate needed detection limits in FCM migrates, we observed that seven of ten genotoxic chemicals would be detectable with HPTLC if present at the regulatory 10 ppb limit and two of ten at TTC for adults. With development, HPTLC-bioassays might become the best option for supporting safety assessment of genotoxicants in food packaging.	de_CH
dc.language.iso	en	de_CH
dc.publisher	Elsevier	de_CH
dc.relation.ispartof	Food Packaging and Shelf Life	de_CH
dc.rights	http://creativecommons.org/licenses/by/4.0/	de_CH
dc.subject	Food contact materials (FCM)	de_CH
dc.subject	High-performance thin layer chromatography (HPTLC)	de_CH
dc.subject	Genotoxicity	de_CH
dc.subject	Threshold of toxicological concern (TTC)	de_CH
dc.subject	Paperboard	de_CH
dc.subject	Non-intentionally added substances (NIAS)	de_CH
dc.subject.ddc	664: Lebensmitteltechnologie	de_CH
dc.title	Towards detecting genotoxic chemicals in food packaging at thresholds of toxicological concern using bioassays with high-performance thin-layer chromatography	de_CH
dc.type	Beitrag in wissenschaftlicher Zeitschrift	de_CH
dcterms.type	Text	de_CH
zhaw.departement	Life Sciences und Facility Management	de_CH
zhaw.organisationalunit	Institut für Umwelt und Natürliche Ressourcen (IUNR)	de_CH
dc.identifier.doi	10.1016/j.fpsl.2023.101052	de_CH
dc.identifier.doi	10.21256/zhaw-27078	-
zhaw.funding.eu	No	de_CH
zhaw.issue	101052	de_CH
zhaw.originated.zhaw	Yes	de_CH
zhaw.publication.status	publishedVersion	de_CH
zhaw.volume	36	de_CH
zhaw.publication.review	Peer review (Publikation)	de_CH
zhaw.webfeed	Ökotechnologie	de_CH
zhaw.author.additional	No	de_CH
zhaw.display.portrait	Yes	de_CH
Appears in collections:	Publikationen Life Sciences und Facility Management

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Bergmann, A. J., Breitenbach, M., Muñoz, C., Simon, E., McCombie, G., Biedermann, M., Schönborn, A., & Vermeirssen, E. L. M. (2023). Towards detecting genotoxic chemicals in food packaging at thresholds of toxicological concern using bioassays with high-performance thin-layer chromatography. Food Packaging and Shelf Life, 36(101052). https://doi.org/10.1016/j.fpsl.2023.101052

Bergmann, A.J. et al. (2023) ‘Towards detecting genotoxic chemicals in food packaging at thresholds of toxicological concern using bioassays with high-performance thin-layer chromatography’, Food Packaging and Shelf Life, 36(101052). Available at: https://doi.org/10.1016/j.fpsl.2023.101052.

A. J. Bergmann et al., “Towards detecting genotoxic chemicals in food packaging at thresholds of toxicological concern using bioassays with high-performance thin-layer chromatography,” Food Packaging and Shelf Life, vol. 36, no. 101052, Apr. 2023, doi: 10.1016/j.fpsl.2023.101052.

BERGMANN, Alan J., Milena BREITENBACH, Celine MUÑOZ, Eszter SIMON, Gregor MCCOMBIE, Maurus BIEDERMANN, Andreas SCHÖNBORN und Etiënne L.M. VERMEIRSSEN, 2023. Towards detecting genotoxic chemicals in food packaging at thresholds of toxicological concern using bioassays with high-performance thin-layer chromatography. Food Packaging and Shelf Life. April 2023. Bd. 36, Nr. 101052. DOI 10.1016/j.fpsl.2023.101052

Bergmann, Alan J., Milena Breitenbach, Celine Muñoz, Eszter Simon, Gregor McCombie, Maurus Biedermann, Andreas Schönborn, and Etiënne L.M. Vermeirssen. 2023. “Towards Detecting Genotoxic Chemicals in Food Packaging at Thresholds of Toxicological Concern Using Bioassays with High-Performance Thin-Layer Chromatography.” Food Packaging and Shelf Life 36 (101052). https://doi.org/10.1016/j.fpsl.2023.101052.

Bergmann, Alan J., et al. “Towards Detecting Genotoxic Chemicals in Food Packaging at Thresholds of Toxicological Concern Using Bioassays with High-Performance Thin-Layer Chromatography.” Food Packaging and Shelf Life, vol. 36, no. 101052, Apr. 2023, https://doi.org/10.1016/j.fpsl.2023.101052.