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https://doi.org/10.21256/zhaw-25992| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Role of HSP70 in response to (thermo)radiotherapy : analysis of gene expression in canine osteosarcoma cells by RNA-seq |
| Autor/-in: | Nytko, Katarzyna J. Thumser-Henner, Pauline Russo, Giancarlo Weyland, Mathias S. Rohrer Bley, Carla |
| et. al: | No |
| DOI: | 10.1038/s41598-020-69619-2 10.21256/zhaw-25992 |
| Erschienen in: | Scientific Reports |
| Band(Heft): | 10 |
| Heft: | 1 |
| Seite(n): | 12779 |
| Erscheinungsdatum: | 2020 |
| Verlag / Hrsg. Institution: | Nature Publishing Group |
| ISSN: | 2045-2322 |
| Sprache: | Englisch |
| Schlagwörter: | Animal; Bone neoplasm; Cell proliferation; Cluster analysis; Disease model, animal; Dog; Gene expression regulation, neoplastic; HSP70 heat-shock protein; Hyperthermia, induced; Matrix metalloproteinase 1; Membrane glycoprotein; Osteosarcoma; Photon; Principal component analysis; RNA, messenger; RNA, small interfering; RNA-seq; Radiotherapy; Vesicular transport protein |
| Fachgebiet (DDC): | 572: Biochemie 615: Pharmakologie und Therapeutik |
| Zusammenfassung: | Pre-treatment of tumors with hyperthermia is often used to increase the efficacy of radiotherapy. One of the main proteins induced in response to hyperthermia is heat shock protein 70 (HSP70). The aim of our study was to investigate up- and down-regulated genes in response to (thermo)radiotherapy in HSP70 proficient and deficient canine osteosarcoma cell line (Abrams), and functional role of HSP70 in the mechanism of thermoradiosensitization. Cells were transfected with negative control siRNA or siRNA targeting HSP70 and treated with hyperthermia (HT), radiotherapy (RT), and thermoradiotherapy (HTRT). RNA sequencing was used to analyze gene expression. Hyperthermia and thermoradiotherapy, but not radiotherapy alone, induced differential gene expression. We identified genes differentially expressed only in HSP70 knockdown (thus HSP70-dependent) cells in response to hyperthermia and thermoradiotherapy. Interestingly, cell proliferation but not clonogenicity and apoptosis/necrosis was affected by the HSP70 knockdown in response to thermoradiotherapy. The results suggest that HSP70 regulates expression of specific genes in response to hyperthermia and thermoradiotherapy. Further investigations into the role of specific genes regulated in a HSP70-dependent manner in response to thermoradiotherapy could pave a way into new, combinatorial treatment options for (canine) osteosarcoma and other cancer types. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/25992 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | CC BY 4.0: Namensnennung 4.0 International |
| Departement: | School of Engineering |
| Organisationseinheit: | Institut für Angewandte Mathematik und Physik (IAMP) |
| Enthalten in den Sammlungen: | Publikationen School of Engineering |
Dateien zu dieser Ressource:
| Datei | Beschreibung | Größe | Format | |
|---|---|---|---|---|
| 2020_Nytko-etal_Role-of-HSP70-inresponse-to-thermoradiotherapie_Sci-Rep.pdf | 1.59 MB | Adobe PDF |  Öffnen/Anzeigen |
Zur Langanzeige
Nytko, K. J., Thumser-Henner, P., Russo, G., Weyland, M. S., & Rohrer Bley, C. (2020). Role of HSP70 in response to (thermo)radiotherapy : analysis of gene expression in canine osteosarcoma cells by RNA-seq. Scientific Reports, 10(1), 12779. https://doi.org/10.1038/s41598-020-69619-2
Nytko, K.J. et al. (2020) ‘Role of HSP70 in response to (thermo)radiotherapy : analysis of gene expression in canine osteosarcoma cells by RNA-seq’, Scientific Reports, 10(1), p. 12779. Available at: https://doi.org/10.1038/s41598-020-69619-2.
K. J. Nytko, P. Thumser-Henner, G. Russo, M. S. Weyland, and C. Rohrer Bley, “Role of HSP70 in response to (thermo)radiotherapy : analysis of gene expression in canine osteosarcoma cells by RNA-seq,” Scientific Reports, vol. 10, no. 1, p. 12779, 2020, doi: 10.1038/s41598-020-69619-2.
NYTKO, Katarzyna J., Pauline THUMSER-HENNER, Giancarlo RUSSO, Mathias S. WEYLAND und Carla ROHRER BLEY, 2020. Role of HSP70 in response to (thermo)radiotherapy : analysis of gene expression in canine osteosarcoma cells by RNA-seq. Scientific Reports. 2020. Bd. 10, Nr. 1, S. 12779. DOI 10.1038/s41598-020-69619-2
Nytko, Katarzyna J., Pauline Thumser-Henner, Giancarlo Russo, Mathias S. Weyland, and Carla Rohrer Bley. 2020. “Role of HSP70 in Response to (Thermo)radiotherapy : Analysis of Gene Expression in Canine Osteosarcoma Cells by RNA-Seq.” Scientific Reports 10 (1): 12779. https://doi.org/10.1038/s41598-020-69619-2.
Nytko, Katarzyna J., et al. “Role of HSP70 in Response to (Thermo)radiotherapy : Analysis of Gene Expression in Canine Osteosarcoma Cells by RNA-Seq.” Scientific Reports, vol. 10, no. 1, 2020, p. 12779, https://doi.org/10.1038/s41598-020-69619-2.
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