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https://doi.org/10.21256/zhaw-25128| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Macromolecular crowding tuned extracellular matrix deposition in a bioprinted human rhabdomyosarcoma model |
| Autor/-in: | D'Agostino, Stefania Rimann, Markus Gamba, Piergiorgio Perilongo, Giorgio Pozzobon, Michela Raghunath, Michael |
| et. al: | No |
| DOI: | 10.1016/j.bprint.2022.e00213 10.21256/zhaw-25128 |
| Erschienen in: | Bioprinting |
| Band(Heft): | 27 |
| Heft: | e00213 |
| Erscheinungsdatum: | 30-Mai-2022 |
| Verlag / Hrsg. Institution: | Elsevier |
| ISSN: | 2405-8866 |
| Sprache: | Englisch |
| Schlagwörter: | Bioprinting; Rhabdomyosarcoma microenvironment; Macromolecular crowding; 3D model of rhabdomyosarcoma |
| Fachgebiet (DDC): | 610.28: Biomedizin, Biomedizinische Technik |
| Zusammenfassung: | The role of the extracellular matrix (ECM) in tumor recurrence and metastasis has been gaining attention. Indeed, not only cellular, but also structural proteins influence migratory and invasive capacity of tumor cells, including growth and resistance to drugs. Therefore, new in vitro tumor models that entail improved ECM formation and deposition are needed. Here, we are developed three-dimensional (3D) models of pediatric soft tissue sarcoma (Rhabdomyosarcoma [RMS]) with the two major subgroups, the embryonal (ERMS) and the alveolar (ARMS) form. We applied macromolecular crowding (MMC) technology to monolayer cultures, spheroids, and 3D bioprinted constructs. In all culture models, exposure to MMC significantly increased ECM deposition. Interestingly, bioprinted constructs showed a collagen and fibronectin matrix architecture that was comparable to that of tumor xenografts. Furthermore, the bioprinted model not only showed tumor cell growth inside the structure but also displayed cell clusters leaving the edges of the bioprinted construct, probably emulating a metastatic mechanism. ARMS and ERMS cells reacted differently in the bioprinted structure. Indeed, the characteristic metastatic behavior was much more pronounced in the more aggressive ARMS subtype. This promising approach opens new avenues for studying RMS microenvironment and creating a platform for cancer drug testing including the native tumor ECM. |
| Weitere Angaben: | EU COST Action CA16119 CellFit |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/25128 |
| Volltext Version: | Akzeptierte Version |
| Lizenz (gemäss Verlagsvertrag): | CC BY-NC-ND 4.0: Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International |
| Gesperrt bis: | 2024-05-26 |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
Dateien zu dieser Ressource:
| Datei | Beschreibung | Größe | Format | |
|---|---|---|---|---|
| 2022_Dagostino-etal_Macromolecular-crowding-extracellular-matrix-deposition-human-rhabdomyosarcoma_AAM.pdf Bis 2024-05-26 | Accepted Version | 1.18 MB | Adobe PDF | Öffnen/Anzeigen |
Zur Langanzeige
D’Agostino, S., Rimann, M., Gamba, P., Perilongo, G., Pozzobon, M., & Raghunath, M. (2022). Macromolecular crowding tuned extracellular matrix deposition in a bioprinted human rhabdomyosarcoma model. Bioprinting, 27(e00213). https://doi.org/10.1016/j.bprint.2022.e00213
D’Agostino, S. et al. (2022) ‘Macromolecular crowding tuned extracellular matrix deposition in a bioprinted human rhabdomyosarcoma model’, Bioprinting, 27(e00213). Available at: https://doi.org/10.1016/j.bprint.2022.e00213.
S. D’Agostino, M. Rimann, P. Gamba, G. Perilongo, M. Pozzobon, and M. Raghunath, “Macromolecular crowding tuned extracellular matrix deposition in a bioprinted human rhabdomyosarcoma model,” Bioprinting, vol. 27, no. e00213, May 2022, doi: 10.1016/j.bprint.2022.e00213.
D’AGOSTINO, Stefania, Markus RIMANN, Piergiorgio GAMBA, Giorgio PERILONGO, Michela POZZOBON und Michael RAGHUNATH, 2022. Macromolecular crowding tuned extracellular matrix deposition in a bioprinted human rhabdomyosarcoma model. Bioprinting. 30 Mai 2022. Bd. 27, Nr. e00213. DOI 10.1016/j.bprint.2022.e00213
D’Agostino, Stefania, Markus Rimann, Piergiorgio Gamba, Giorgio Perilongo, Michela Pozzobon, and Michael Raghunath. 2022. “Macromolecular Crowding Tuned Extracellular Matrix Deposition in a Bioprinted Human Rhabdomyosarcoma Model.” Bioprinting 27 (e00213). https://doi.org/10.1016/j.bprint.2022.e00213.
D’Agostino, Stefania, et al. “Macromolecular Crowding Tuned Extracellular Matrix Deposition in a Bioprinted Human Rhabdomyosarcoma Model.” Bioprinting, vol. 27, no. e00213, May 2022, https://doi.org/10.1016/j.bprint.2022.e00213.
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