1. ZHAW digitalcollection
  2. Life Sciences und Facility Management
  3. Publikationen
  4. Publikationen Life Sciences und Facility Management
Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-23220
Full metadata record
DC FieldValueLanguage
dc.contributor.authorKurz, Mirjam-
dc.contributor.authorCarnal, Simon-
dc.contributor.authorDafny-Yelin, Mery-
dc.contributor.authorMairesse, Orly-
dc.contributor.authorGottsberger, Richard A.-
dc.contributor.authorIvanović, Milan-
dc.contributor.authorGrahovac, Mila-
dc.contributor.authorLagonenko, Alexander L.-
dc.contributor.authorDrenova, Nataliya-
dc.contributor.authorZharmukhamedova, Galiya-
dc.contributor.authorDoolotkeldieva, Tinatin-
dc.contributor.authorSmits, Theo H.M.-
dc.contributor.authorRezzonico, Fabio-
dc.date.accessioned2021-09-27T13:40:00Z-
dc.date.available2021-09-27T13:40:00Z-
dc.date.issued2021-08-04-
dc.identifier.issn2524-4167de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/23220-
dc.description.abstractFire blight is the most devastating disease affecting pome fruit production globally. The pathogen is native to North America and was imported to western Europe in the 1950s, progressively spreading over the continent in theensuing decades. Previous phylogenetic studies have revealed the extreme genetic homogeneity of the pathogenoutside its center of origin, which makes epidemiological studies difficult. These are generally only possible using hypervariable regions of the genome such as those represented by CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats), which are, however, not practical to sequence due to their size and variability. Here, we present a simple PCR assay targeting the duplication of a single CRISPR spacer in Erwinia amylovora that was found to be an important marker to discriminate between two main European populations of the pathogen. We implemented the assay on a total of 582 isolates to follow the spread of fire blight across the continent over several decades and, wherever possible, within single countries. The results obtained point to the occurrence of two major separate introduction events for E. amylovora in Europe that occurred approximately 20 years apart, and confirmed the existence of two principal distribution areas located in Northeastern Europe and the Eastern Mediterranean Basin from which the pathogen moved on to colonize the Eurasian continent.de_CH
dc.language.isoende_CH
dc.publisherBioMed Centralde_CH
dc.relation.ispartofPhytopathology Researchde_CH
dc.rightshttp://creativecommons.org/licenses/by/4.0/de_CH
dc.subjectPopulation geneticsde_CH
dc.subjectFounder genotypede_CH
dc.subjectCentral Asiade_CH
dc.subjectWild apple forestde_CH
dc.subject.ddc572: Biochemiede_CH
dc.subject.ddc632: Pflanzenkrankheiten, Schädlingede_CH
dc.titleTracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacerde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Umwelt und Natürliche Ressourcen (IUNR)de_CH
dc.identifier.doi10.1186/s42483-021-00096-9de_CH
dc.identifier.doi10.21256/zhaw-23220-
zhaw.funding.euNode_CH
zhaw.issue18de_CH
zhaw.originated.zhawYesde_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume3de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.funding.snf177515de_CH
zhaw.webfeedUmweltgenomikde_CH
zhaw.funding.zhawSchutz von Apfelwälderökosystemen in Zentralasien vor dem bakteriellen Pathogen Erwinia amylovora (Feuerbrand)de_CH
zhaw.author.additionalNode_CH
zhaw.display.portraitYesde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

Files in This Item:
File Description SizeFormat 
2021_Kurz-etal_Tracking-dissemination-Erwinia-amylovora.pdf2.62 MBAdobe PDFThumbnail
View/Open
Show simple item record
Kurz, M., Carnal, S., Dafny-Yelin, M., Mairesse, O., Gottsberger, R. A., Ivanović, M., Grahovac, M., Lagonenko, A. L., Drenova, N., Zharmukhamedova, G., Doolotkeldieva, T., Smits, T. H. M., & Rezzonico, F. (2021). Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer. Phytopathology Research, 3(18). https://doi.org/10.1186/s42483-021-00096-9
Kurz, M. et al. (2021) ‘Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer’, Phytopathology Research, 3(18). Available at: https://doi.org/10.1186/s42483-021-00096-9.
M. Kurz et al., “Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer,” Phytopathology Research, vol. 3, no. 18, Aug. 2021, doi: 10.1186/s42483-021-00096-9.
KURZ, Mirjam, Simon CARNAL, Mery DAFNY-YELIN, Orly MAIRESSE, Richard A. GOTTSBERGER, Milan IVANOVIĆ, Mila GRAHOVAC, Alexander L. LAGONENKO, Nataliya DRENOVA, Galiya ZHARMUKHAMEDOVA, Tinatin DOOLOTKELDIEVA, Theo H.M. SMITS und Fabio REZZONICO, 2021. Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer. Phytopathology Research. 4 August 2021. Bd. 3, Nr. 18. DOI 10.1186/s42483-021-00096-9
Kurz, Mirjam, Simon Carnal, Mery Dafny-Yelin, Orly Mairesse, Richard A. Gottsberger, Milan Ivanović, Mila Grahovac, et al. 2021. “Tracking the Dissemination of Erwinia Amylovora in the Eurasian Continent Using a PCR Targeted on the Duplication of a Single CRISPR Spacer.” Phytopathology Research 3 (18). https://doi.org/10.1186/s42483-021-00096-9.
Kurz, Mirjam, et al. “Tracking the Dissemination of Erwinia Amylovora in the Eurasian Continent Using a PCR Targeted on the Duplication of a Single CRISPR Spacer.” Phytopathology Research, vol. 3, no. 18, Aug. 2021, https://doi.org/10.1186/s42483-021-00096-9.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.