Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer

Kurz, Mirjam; Carnal, Simon; Dafny-Yelin, Mery; Mairesse, Orly; Gottsberger, Richard A.; Ivanović, Milan; Grahovac, Mila; Lagonenko, Alexander L.; Drenova, Nataliya; Zharmukhamedova, Galiya; Doolotkeldieva, Tinatin; Smits, Theo H.M.; Rezzonico, Fabio

doi:10.1186/s42483-021-00096-9

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-23220

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer
Authors:	Kurz, Mirjam Carnal, Simon Dafny-Yelin, Mery Mairesse, Orly Gottsberger, Richard A. Ivanović, Milan Grahovac, Mila Lagonenko, Alexander L. Drenova, Nataliya Zharmukhamedova, Galiya Doolotkeldieva, Tinatin Smits, Theo H.M. Rezzonico, Fabio
et. al:	No
DOI:	10.1186/s42483-021-00096-9 10.21256/zhaw-23220
Published in:	Phytopathology Research
Volume(Issue):	3
Issue:	18
Issue Date:	4-Aug-2021
Publisher / Ed. Institution:	BioMed Central
ISSN:	2524-4167
Language:	English
Subjects:	Population genetics; Founder genotype; Central Asia; Wild apple forest
Subject (DDC):	572: Biochemistry 632: Plant diseases, pests
Abstract:	Fire blight is the most devastating disease affecting pome fruit production globally. The pathogen is native to North America and was imported to western Europe in the 1950s, progressively spreading over the continent in theensuing decades. Previous phylogenetic studies have revealed the extreme genetic homogeneity of the pathogenoutside its center of origin, which makes epidemiological studies difficult. These are generally only possible using hypervariable regions of the genome such as those represented by CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats), which are, however, not practical to sequence due to their size and variability. Here, we present a simple PCR assay targeting the duplication of a single CRISPR spacer in Erwinia amylovora that was found to be an important marker to discriminate between two main European populations of the pathogen. We implemented the assay on a total of 582 isolates to follow the spread of fire blight across the continent over several decades and, wherever possible, within single countries. The results obtained point to the occurrence of two major separate introduction events for E. amylovora in Europe that occurred approximately 20 years apart, and confirmed the existence of two principal distribution areas located in Northeastern Europe and the Eastern Mediterranean Basin from which the pathogen moved on to colonize the Eurasian continent.
URI:	https://digitalcollection.zhaw.ch/handle/11475/23220
Fulltext version:	Published version
License (according to publishing contract):	CC BY 4.0: Attribution 4.0 International
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Natural Resource Sciences (IUNR)
Published as part of the ZHAW project:	Schutz von Apfelwälderökosystemen in Zentralasien vor dem bakteriellen Pathogen Erwinia amylovora (Feuerbrand)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Kurz, M., Carnal, S., Dafny-Yelin, M., Mairesse, O., Gottsberger, R. A., Ivanović, M., Grahovac, M., Lagonenko, A. L., Drenova, N., Zharmukhamedova, G., Doolotkeldieva, T., Smits, T. H. M., & Rezzonico, F. (2021). Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer. Phytopathology Research, 3(18). https://doi.org/10.1186/s42483-021-00096-9

Kurz, M. et al. (2021) ‘Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer’, Phytopathology Research, 3(18). Available at: https://doi.org/10.1186/s42483-021-00096-9.

M. Kurz et al., “Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer,” Phytopathology Research, vol. 3, no. 18, Aug. 2021, doi: 10.1186/s42483-021-00096-9.

KURZ, Mirjam, Simon CARNAL, Mery DAFNY-YELIN, Orly MAIRESSE, Richard A. GOTTSBERGER, Milan IVANOVIĆ, Mila GRAHOVAC, Alexander L. LAGONENKO, Nataliya DRENOVA, Galiya ZHARMUKHAMEDOVA, Tinatin DOOLOTKELDIEVA, Theo H.M. SMITS und Fabio REZZONICO, 2021. Tracking the dissemination of Erwinia amylovora in the Eurasian continent using a PCR targeted on the duplication of a single CRISPR spacer. Phytopathology Research. 4 August 2021. Bd. 3, Nr. 18. DOI 10.1186/s42483-021-00096-9

Kurz, Mirjam, Simon Carnal, Mery Dafny-Yelin, Orly Mairesse, Richard A. Gottsberger, Milan Ivanović, Mila Grahovac, et al. 2021. “Tracking the Dissemination of Erwinia Amylovora in the Eurasian Continent Using a PCR Targeted on the Duplication of a Single CRISPR Spacer.” Phytopathology Research 3 (18). https://doi.org/10.1186/s42483-021-00096-9.

Kurz, Mirjam, et al. “Tracking the Dissemination of Erwinia Amylovora in the Eurasian Continent Using a PCR Targeted on the Duplication of a Single CRISPR Spacer.” Phytopathology Research, vol. 3, no. 18, Aug. 2021, https://doi.org/10.1186/s42483-021-00096-9.