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https://doi.org/10.21256/zhaw-21975| Publikationstyp: | Buchbeitrag |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Numerical methods for the design and description of in vitro expansion processes of human mesenchymal stem cells |
| Autor/-in: | Jossen, Valentin Eibl, Dieter Eibl, Regine |
| et. al: | No |
| DOI: | 10.1007/10_2020_147 10.21256/zhaw-21975 |
| Erschienen in: | Digital Twins |
| Seite(n): | 185 |
| Seiten bis: | 228 |
| Erscheinungsdatum: | 23-Okt-2020 |
| Reihe: | Advances in Biochemical Engineering / Biotechnology |
| Reihenzählung: | 177 |
| Verlag / Hrsg. Institution: | Springer |
| Verlag / Hrsg. Institution: | Berlin |
| Sprache: | Englisch |
| Schlagwörter: | Computational fluid dynamics; Euler-Euler model; Euler-Lagrange model; Human mesenchymal stem cell; Kinetic growth modelling; Microcarrier technology |
| Fachgebiet (DDC): | 660.6: Biotechnologie |
| Zusammenfassung: | Human mesenchymal stem cells (hMSCs) are a valuable source of cells for clinical applications (e.g., treatment of acute myocardial infarction or inflammatory diseases), especially in the field of regenerative medicine. However, for autologous (patient-specific) and allogeneic (off-the-shelf) hMSC-based therapies, in vitro expansion is necessary prior to the clinical application in order to achieve the required cell numbers. Safe, reproducible, and economic in vitro expansion of hMSCs for autologous and allogeneic therapies can be problematic because the cell material is restricted and the cells are sensitive to environmental changes. It is beneficial to collect detailed information on the hydrodynamic conditions and cell growth behavior in a bioreactor system, in order to develop a so called “Digital Twin” of the cultivation system and expansion process. Numerical methods, such as Computational Fluid Dynamics (CFD) which has become widely used in the biotech industry for studying local characteristics within bioreactors or kinetic growth modelling, provide possible solutions for such tasks. In this review, we will present the current state-of-the-art for the in vitro expansion of hMSCs. Different numerical tools, including numerical fluid flow simulations and cell growth modelling approaches for hMSCs, will be presented. In addition, a case study demonstrating the applicability of CFD and kinetic growth modelling for the development of an microcarrier-based hMSC process will be shown. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/21975 |
| Volltext Version: | Akzeptierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Gesperrt bis: | 2021-10-23 |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
Dateien zu dieser Ressource:
| Datei | Beschreibung | Größe | Format | |
|---|---|---|---|---|
| 2020_Jossen-etal_In-vitro-expansion-mesenchymal-stem-cells.pdf | Accepted Version | 1.64 MB | Adobe PDF |  Öffnen/Anzeigen |
Zur Langanzeige
Jossen, V., Eibl, D., & Eibl, R. (2020). Numerical methods for the design and description of in vitro expansion processes of human mesenchymal stem cells. In Digital Twins (pp. 185–228). Springer. https://doi.org/10.1007/10_2020_147
Jossen, V., Eibl, D. and Eibl, R. (2020) ‘Numerical methods for the design and description of in vitro expansion processes of human mesenchymal stem cells’, in Digital Twins. Berlin: Springer, pp. 185–228. Available at: https://doi.org/10.1007/10_2020_147.
V. Jossen, D. Eibl, and R. Eibl, “Numerical methods for the design and description of in vitro expansion processes of human mesenchymal stem cells,” in Digital Twins, Berlin: Springer, 2020, pp. 185–228. doi: 10.1007/10_2020_147.
JOSSEN, Valentin, Dieter EIBL und Regine EIBL, 2020. Numerical methods for the design and description of in vitro expansion processes of human mesenchymal stem cells. In: Digital Twins. Berlin: Springer. S. 185–228
Jossen, Valentin, Dieter Eibl, and Regine Eibl. 2020. “Numerical Methods for the Design and Description of in Vitro Expansion Processes of Human Mesenchymal Stem Cells.” In Digital Twins, 185–228. Berlin: Springer. https://doi.org/10.1007/10_2020_147.
Jossen, Valentin, et al. “Numerical Methods for the Design and Description of in Vitro Expansion Processes of Human Mesenchymal Stem Cells.” Digital Twins, Springer, 2020, pp. 185–228, https://doi.org/10.1007/10_2020_147.
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