Bitte benutzen Sie diese Kennung, um auf die Ressource zu verweisen:
https://doi.org/10.21256/zhaw-3500| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Comparison of plant-based expression platforms for the heterologous production of geraniol |
| Autor/-in: | Vasilev, Nikolay Schmitz, Christian Dong, Lemeng Ritala, Anneli Imseng, Nicole Häkkinen, Suvi T. van der Krol, Sander Eibl-Schindler, Regine Oksman-Caldentey, Kirsi-Marja Bouwmeester, Harro Fischer, Rainer Schillberg, Stefan |
| DOI: | 10.1007/s11240-014-0446-z 10.21256/zhaw-3500 |
| Erschienen in: | Plant Cell, Tissue and Organ Culture (PCTOC) |
| Band(Heft): | 117 |
| Heft: | 3 |
| Seite(n): | 373 |
| Seiten bis: | 380 |
| Erscheinungsdatum: | 2014 |
| Verlag / Hrsg. Institution: | Springer |
| ISSN: | 0167-6857 1573-5044 |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 660.6: Biotechnologie |
| Zusammenfassung: | We compared the ability of different plant-based expression platforms to produce geraniol, a key metabolite in the monoterpenoid branch of the terpenoid indole alkaloid biosynthesis pathway. A geraniol synthase gene isolated from Valeriana officinalis (VoGES) was stably expressed in different tobacco systems. Intact plants were grown in vitro and in the greenhouse and were used to generate cell suspension and hairy root cultures. VoGES was also transiently expressed in N. benthamiana. The highest geraniol content was produced by intact transgenic plants grown in vitro (48 µg/g fresh weight, fw), followed by the transient expression system (27 µg/g fw), transgenic plants under hydroponic conditions in the greenhouse and cell suspension cultures (16 µg/g fw), and finally hairy root cultures (9 µg/g fw). Differences in biomass production and the duration of cultivation resulted in a spectrum of geraniol productivities. Cell suspension cultures achieved a geraniol production rate of 1.8 µg/g fresh biomass per day, whereas transient expression produced 5.9 µg/g fresh biomass per day (if cultivation prior to agroinfiltration is ignored) or 0.5 µg/g fresh biomass per day (if cultivation prior to agroinfiltration is included). The superior productivity, strict process control and simple handling procedures available for transgenic cell suspension cultures suggest that cells are the most promising system for further optimization and ultimately for the scaled up production of geraniol. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/2075 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Gesperrt bis: | 2020-01-01 |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
Dateien zu dieser Ressource:
| Datei | Beschreibung | Größe | Format | |
|---|---|---|---|---|
| 10.1007_s11240-014-0446-z.pdf | 292.79 kB | Adobe PDF |  Öffnen/Anzeigen |
Zur Langanzeige
Vasilev, N., Schmitz, C., Dong, L., Ritala, A., Imseng, N., Häkkinen, S. T., van der Krol, S., Eibl-Schindler, R., Oksman-Caldentey, K.-M., Bouwmeester, H., Fischer, R., & Schillberg, S. (2014). Comparison of plant-based expression platforms for the heterologous production of geraniol. Plant Cell, Tissue and Organ Culture (PCTOC), 117(3), 373–380. https://doi.org/10.1007/s11240-014-0446-z
Vasilev, N. et al. (2014) ‘Comparison of plant-based expression platforms for the heterologous production of geraniol’, Plant Cell, Tissue and Organ Culture (PCTOC), 117(3), pp. 373–380. Available at: https://doi.org/10.1007/s11240-014-0446-z.
N. Vasilev et al., “Comparison of plant-based expression platforms for the heterologous production of geraniol,” Plant Cell, Tissue and Organ Culture (PCTOC), vol. 117, no. 3, pp. 373–380, 2014, doi: 10.1007/s11240-014-0446-z.
VASILEV, Nikolay, Christian SCHMITZ, Lemeng DONG, Anneli RITALA, Nicole IMSENG, Suvi T. HÄKKINEN, Sander VAN DER KROL, Regine EIBL-SCHINDLER, Kirsi-Marja OKSMAN-CALDENTEY, Harro BOUWMEESTER, Rainer FISCHER und Stefan SCHILLBERG, 2014. Comparison of plant-based expression platforms for the heterologous production of geraniol. Plant Cell, Tissue and Organ Culture (PCTOC). 2014. Bd. 117, Nr. 3, S. 373–380. DOI 10.1007/s11240-014-0446-z
Vasilev, Nikolay, Christian Schmitz, Lemeng Dong, Anneli Ritala, Nicole Imseng, Suvi T. Häkkinen, Sander van der Krol, et al. 2014. “Comparison of Plant-Based Expression Platforms for the Heterologous Production of Geraniol.” Plant Cell, Tissue and Organ Culture (PCTOC) 117 (3): 373–80. https://doi.org/10.1007/s11240-014-0446-z.
Vasilev, Nikolay, et al. “Comparison of Plant-Based Expression Platforms for the Heterologous Production of Geraniol.” Plant Cell, Tissue and Organ Culture (PCTOC), vol. 117, no. 3, 2014, pp. 373–80, https://doi.org/10.1007/s11240-014-0446-z.
Alle Ressourcen in diesem Repository sind urheberrechtlich geschützt, soweit nicht anderweitig angezeigt.