| Publikationstyp: | Konferenz: Sonstiges |
| Art der Begutachtung: | Peer review (Abstract) |
| Titel: | Enhanced process insights in shake flask cultivations |
| Autor/-in: | John, Gernot. T Maschke, Rüdiger |
| et. al: | No |
| Angaben zur Konferenz: | BioTech 2019 : Biopharmaceutical Manufacturing and Single-Use Technologies, Wädenswil, Switzerland, 2-4 July 2019 |
| Erscheinungsdatum: | Jul-2019 |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 540: Chemie |
| Zusammenfassung: | While sophisticated small-scale cultivation systems came to the market in the last few years, shake flasks maintain the economic alternative widely used in industry and academia. Yet, these vessels lack the sensors like turbidity, oxygen concentration and pH-values. Here we show how to overcome these hurdles. The first part covers measurement improvements for shake flasks with focus on back-scattered light for biomass growth monitoring and the utilization of sensor spots for a deeper insight into the “black box” shake flasks. We used plant cells as their growth often can’t be accurately monitored by simple offline OD measurements. The sensor spot measurements are further used to detect process events, enabling the prediction of metabolite depletion. In the second part, soft sensors based on engineering and biological characterizations of these vessels are introduced and explained, enabling researchers to predict process values and estimate growth curves. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/19535 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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John, Gernot. T., & Maschke, R. (2019, July). Enhanced process insights in shake flask cultivations. BioTech 2019 : Biopharmaceutical Manufacturing and Single-Use Technologies, Wädenswil, Switzerland, 2-4 July 2019.
John, Gernot.T. and Maschke, R. (2019) ‘Enhanced process insights in shake flask cultivations’, in BioTech 2019 : Biopharmaceutical Manufacturing and Single-Use Technologies, Wädenswil, Switzerland, 2-4 July 2019.
Gernot. T. John and R. Maschke, “Enhanced process insights in shake flask cultivations,” in BioTech 2019 : Biopharmaceutical Manufacturing and Single-Use Technologies, Wädenswil, Switzerland, 2-4 July 2019, Jul. 2019.
JOHN, Gernot. T und Rüdiger MASCHKE, 2019. Enhanced process insights in shake flask cultivations. In: BioTech 2019 : Biopharmaceutical Manufacturing and Single-Use Technologies, Wädenswil, Switzerland, 2-4 July 2019. Conference presentation. Juli 2019
John, Gernot. T, and Rüdiger Maschke. 2019. “Enhanced Process Insights in Shake Flask Cultivations.” Conference presentation. In BioTech 2019 : Biopharmaceutical Manufacturing and Single-Use Technologies, Wädenswil, Switzerland, 2-4 July 2019.
John, Gernot. T., and Rüdiger Maschke. “Enhanced Process Insights in Shake Flask Cultivations.” BioTech 2019 : Biopharmaceutical Manufacturing and Single-Use Technologies, Wädenswil, Switzerland, 2-4 July 2019, 2019.
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