| Publikationstyp: | Konferenz: Poster |
| Art der Begutachtung: | Peer review (Abstract) |
| Titel: | Screening for active substances with TLC bioautography : challenges with artefacts |
| Autor/-in: | Merki, Rebekka Schönborn, Andreas Pihan, Lisa-Anna-Maria Wolfram, Evelyn |
| et. al: | No |
| Tagungsband: | 12th BioDetectors Conference 2019 : Abstract book |
| Angaben zur Konferenz: | 12th BioDetectors Conference 2019, Vienna, Austria, 26-27 September 2019 |
| Erscheinungsdatum: | 2019 |
| Verlag / Hrsg. Institution: | BioDetection Systems |
| Verlag / Hrsg. Institution: | Vienna |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 540: Chemie |
| Zusammenfassung: | TLC, and its modern form HPTLC, is a standard method for identification and quality control of herbals [1]. Its combination with bioassays offers simultaneous separation and screening of plant extracts and other multi-compound mixtures for active substances. The intriguing simplicity and rapidness of the method might lead to underestimation of potential artefacts, first reported by Taibon et al. for HPTLC-Tyrosinase Inhibition [2]. In assays based on cells or enzymes in hydrophilic media, high lipophilic substances prevent contact with the potentially active substance and might lead to false-positive or false-negative results. Additionally, diffusion problems from the layer to the biological assay agents can also lead to wrong interpretation of results, due to difference in the physico-chemical properties of the molecules in the sample mixture. The planar Yeast Estrogen Screen Assay (pYES) is used for detection of human estrogen receptor alpha agonists in mixtures [3]. The hER-alpha is expressing beta-galactosidase in presence of estrogenic substances. Substances with a strong blue autofluorescence at 366 nm detection and low estrogenic activity are difficult to differentiate from real estrogenic activity, when using the blue fluorescent dye 4-methylumbelliferyl-beta-D-galactopyranosid (MUG) as substarte for beta galactosidase. Results of blue fluorescing phenolic acid rich plant extracts with known estrogen activity are demonstrated and a control assay using beta-D-galactopyranoside (RGP) as substrate is proposed [4]. The poster summarizes potential artefacts from pYES and other antimicrobial assays and reviews the theoretical considerations and suggests further investigation to overcome the challenges and improve bioautographic methods. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/19502 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Keine Angabe |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Zur Langanzeige
Merki, R., Schönborn, A., Pihan, L.-A.-M., & Wolfram, E. (2019). Screening for active substances with TLC bioautography : challenges with artefacts. 12th BioDetectors Conference 2019 : Abstract Book.
Merki, R. et al. (2019) ‘Screening for active substances with TLC bioautography : challenges with artefacts’, in 12th BioDetectors Conference 2019 : Abstract book. Vienna: BioDetection Systems.
R. Merki, A. Schönborn, L.-A.-M. Pihan, and E. Wolfram, “Screening for active substances with TLC bioautography : challenges with artefacts,” in 12th BioDetectors Conference 2019 : Abstract book, 2019.
MERKI, Rebekka, Andreas SCHÖNBORN, Lisa-Anna-Maria PIHAN und Evelyn WOLFRAM, 2019. Screening for active substances with TLC bioautography : challenges with artefacts. In: 12th BioDetectors Conference 2019 : Abstract book. Conference poster. Vienna: BioDetection Systems. 2019
Merki, Rebekka, Andreas Schönborn, Lisa-Anna-Maria Pihan, and Evelyn Wolfram. 2019. “Screening for Active Substances with TLC Bioautography : Challenges with Artefacts.” Conference poster. In 12th BioDetectors Conference 2019 : Abstract Book. Vienna: BioDetection Systems.
Merki, Rebekka, et al. “Screening for Active Substances with TLC Bioautography : Challenges with Artefacts.” 12th BioDetectors Conference 2019 : Abstract Book, BioDetection Systems, 2019.
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