| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Incorporation of a prolyl hydroxylase inhibitor into scaffolds : a strategy for stimulating vascularization |
| Autor/-in: | Sham, Adeline Martinez, Eliana C. Beyer, Sebastian Trau, Dieter W. Raghunath, Michael |
| DOI: | 10.1089/ten.TEA.2014.0077 |
| Erschienen in: | Tissue Engineering - Part A |
| Band(Heft): | 21 |
| Heft: | 5-6 |
| Erscheinungsdatum: | 2015 |
| Verlag / Hrsg. Institution: | Mary Ann Liebert |
| ISSN: | 1937-335X 1937-3341 |
| Sprache: | Englisch |
| Fachgebiet (DDC): | 571: Physiologie und verwandte Themen 616: Innere Medizin und Krankheiten |
| Zusammenfassung: | Clinical applications of tissue engineering are constrained by the ability of the implanted construct to invoke vascularization in adequate extent and velocity. To overcome the current limitations presented by local delivery of single angiogenic factors, we explored the incorporation of prolyl hydroxylase inhibitors (PHIs) into scaffolds as an alternative vascularization strategy. PHIs are small molecule drugs that can stabilize the alpha subunit of hypoxia-inducible factor-1 (HIF-1), a key transcription factor that regulates a variety of angiogenic mechanisms. In this study, we conjugated the PHI pyridine-2,4-dicarboxylic acid (PDCA) through amide bonds to a gelatin sponge (Gelfoam®). Fibroblasts cultured on PDCA-Gelfoam were able to infiltrate and proliferate in these scaffolds while secreting significantly more vascular endothelial growth factor than cells grown on Gelfoam without PDCA. Reporter cells expressing green fluorescent protein-tagged HIF-1α exhibited dose-dependent stabilization of this angiogenic transcription factor when growing within PDCA-Gelfoam constructs. Subsequently, we implanted PDCA-Gelfoam scaffolds into the perirenal fat tissue of Sprague Dawley rats for 8 days. Immunostaining of explants revealed that the PDCA-Gelfoam scaffolds were amply infiltrated by cells and promoted vascular ingrowth in a dose-dependent manner. Thus, the incorporation of PHIs into scaffolds appears to be a feasible strategy for improving vascularization in regenerative medicine applications. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/12191 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Sham, A., Martinez, E. C., Beyer, S., Trau, D. W., & Raghunath, M. (2015). Incorporation of a prolyl hydroxylase inhibitor into scaffolds : a strategy for stimulating vascularization. Tissue Engineering - Part A, 21(5-6). https://doi.org/10.1089/ten.TEA.2014.0077
Sham, A. et al. (2015) ‘Incorporation of a prolyl hydroxylase inhibitor into scaffolds : a strategy for stimulating vascularization’, Tissue Engineering - Part A, 21(5-6). Available at: https://doi.org/10.1089/ten.TEA.2014.0077.
A. Sham, E. C. Martinez, S. Beyer, D. W. Trau, and M. Raghunath, “Incorporation of a prolyl hydroxylase inhibitor into scaffolds : a strategy for stimulating vascularization,” Tissue Engineering - Part A, vol. 21, no. 5-6, 2015, doi: 10.1089/ten.TEA.2014.0077.
SHAM, Adeline, Eliana C. MARTINEZ, Sebastian BEYER, Dieter W. TRAU und Michael RAGHUNATH, 2015. Incorporation of a prolyl hydroxylase inhibitor into scaffolds : a strategy for stimulating vascularization. Tissue Engineering - Part A. 2015. Bd. 21, Nr. 5-6. DOI 10.1089/ten.TEA.2014.0077
Sham, Adeline, Eliana C. Martinez, Sebastian Beyer, Dieter W. Trau, and Michael Raghunath. 2015. “Incorporation of a Prolyl Hydroxylase Inhibitor into Scaffolds : A Strategy for Stimulating Vascularization.” Tissue Engineering - Part A 21 (5-6). https://doi.org/10.1089/ten.TEA.2014.0077.
Sham, Adeline, et al. “Incorporation of a Prolyl Hydroxylase Inhibitor into Scaffolds : A Strategy for Stimulating Vascularization.” Tissue Engineering - Part A, vol. 21, no. 5-6, 2015, https://doi.org/10.1089/ten.TEA.2014.0077.
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