Publication type: | Article in scientific journal |
Type of review: | Peer review (publication) |
Title: | Sourcing of an alternative pericyte-like cell type from peripheral blood generated with pulsed macromolecular crowding |
Authors: | Blocki, Anna Wang, Yingting Koch, Maria Goralczyk, Anna Beyer, Sebastian Agarwal, Nikita Lee, Michelle Moonshi, Shehzahdi Dewavrin, Jean-Yves Peh, Priscilla Schwarz, Herbert Bhakoo, Kishore Raghunath, Michael |
DOI: | 10.1038/mt.2014.232 |
Published in: | Molecular Therapy |
Volume(Issue): | 23 |
Issue: | 3 |
Page(s): | 510 |
Pages to: | 522 |
Issue Date: | 2015 |
Publisher / Ed. Institution: | Elsevier |
ISSN: | 1525-0016 1525-0024 |
Language: | English |
Subject (DDC): | 610: Medicine and health 615: Pharmacology and therapeutics |
Abstract: | Autologous cells hold great potential for personalized cell therapy, reducing immunological and risk of infections. However, low cell counts at harvest with subsequently long expansion times with associated cell function loss currently impede the advancement of autologous cell therapy approaches. Here, we aimed to source clinically relevant numbers of proangiogenic cells from an easy accessible cell source, namely peripheral blood. Using macromolecular crowding (MMC) as a biotechnological platform, we derived a novel cell type from peripheral blood that is generated within 5 days in large numbers (10-40 million cells per 100 ml of blood). This blood-derived angiogenic cell (BDAC) type is of monocytic origin, but exhibits pericyte markers PDGFR-β and NG2 and demonstrates strong angiogenic activity, hitherto ascribed only to MSC-like pericytes. Our findings suggest that BDACs represent an alternative pericyte-like cell population of hematopoietic origin that is involved in promoting early stages of microvasculature formation. As a proof of principle of BDAC efficacy in an ischemic disease model, BDAC injection rescued affected tissues in a murine hind limb ischemia model by accelerating and enhancing revascularization. Derived from a renewable tissue that is easy to collect, BDACs overcome current short-comings of autologous cell therapy, in particular for tissue repair strategies. |
URI: | https://digitalcollection.zhaw.ch/handle/11475/12182 |
Fulltext version: | Published version |
License (according to publishing contract): | Licence according to publishing contract |
Departement: | Life Sciences and Facility Management |
Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
Appears in collections: | Publikationen Life Sciences und Facility Management |
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Blocki, A., Wang, Y., Koch, M., Goralczyk, A., Beyer, S., Agarwal, N., Lee, M., Moonshi, S., Dewavrin, J.-Y., Peh, P., Schwarz, H., Bhakoo, K., & Raghunath, M. (2015). Sourcing of an alternative pericyte-like cell type from peripheral blood generated with pulsed macromolecular crowding. Molecular Therapy, 23(3), 510–522. https://doi.org/10.1038/mt.2014.232
Blocki, A. et al. (2015) ‘Sourcing of an alternative pericyte-like cell type from peripheral blood generated with pulsed macromolecular crowding’, Molecular Therapy, 23(3), pp. 510–522. Available at: https://doi.org/10.1038/mt.2014.232.
A. Blocki et al., “Sourcing of an alternative pericyte-like cell type from peripheral blood generated with pulsed macromolecular crowding,” Molecular Therapy, vol. 23, no. 3, pp. 510–522, 2015, doi: 10.1038/mt.2014.232.
BLOCKI, Anna, Yingting WANG, Maria KOCH, Anna GORALCZYK, Sebastian BEYER, Nikita AGARWAL, Michelle LEE, Shehzahdi MOONSHI, Jean-Yves DEWAVRIN, Priscilla PEH, Herbert SCHWARZ, Kishore BHAKOO und Michael RAGHUNATH, 2015. Sourcing of an alternative pericyte-like cell type from peripheral blood generated with pulsed macromolecular crowding. Molecular Therapy. 2015. Bd. 23, Nr. 3, S. 510–522. DOI 10.1038/mt.2014.232
Blocki, Anna, Yingting Wang, Maria Koch, Anna Goralczyk, Sebastian Beyer, Nikita Agarwal, Michelle Lee, et al. 2015. “Sourcing of an Alternative Pericyte-like Cell Type from Peripheral Blood Generated with Pulsed Macromolecular Crowding.” Molecular Therapy 23 (3): 510–22. https://doi.org/10.1038/mt.2014.232.
Blocki, Anna, et al. “Sourcing of an Alternative Pericyte-like Cell Type from Peripheral Blood Generated with Pulsed Macromolecular Crowding.” Molecular Therapy, vol. 23, no. 3, 2015, pp. 510–22, https://doi.org/10.1038/mt.2014.232.
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