| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Flow-cytometric detection of changes in the physiological state of E. coli expressing a heterologous membrane protein during carbon-limited fedbatch cultivation |
| Autor/-in: | Looser, Verena Hammes, Frederik Keller, Markus Berney, Michael Kovar, Karin Egli, Thomas |
| DOI: | 10.1002/bit.20575 |
| Erschienen in: | Biotechnology & Bioengineering |
| Band(Heft): | 92 |
| Heft: | 1 |
| Seite(n): | 69 |
| Seiten bis: | 78 |
| Erscheinungsdatum: | 5-Okt-2005 |
| Verlag / Hrsg. Institution: | Wiley |
| ISSN: | 1097-0290 0006-3592 |
| Sprache: | Englisch |
| Schlagwörter: | Ethidium bromide; Fedbatch; E. coli; Flow cytometry; Physiological state; Propidium iodide; Recombinant membrane protein; Xylene monooxygenase |
| Fachgebiet (DDC): | 660.6: Biotechnologie |
| Zusammenfassung: | The key to optimizing productivity during industrial fermentations is the ability to rapidly monitor and interpret the physiological state of single microbial cells in a population and to recognize and characterize different sub-populations. Here, a flow cytometry-based method for the reproducible detection of changes in membrane function and/or structure of recombinant E. coli JM101 (pSPZ3) expressing xylene monooxygenase (XMO), was developed. XMO expression led to compromised but not permeabilized cell membranes. This was deduced from the fact that recombinant cells only stained with ethidium bromide (EB) and not with propidium iodide (PI). During the glucose-limited fedbatch cultivation, an increase from 25% to 95% of EB-stained cells was observed, occurring between 2 and 5 h after induction. Control experiments confirmed that this increase was due to the recombinant protein production and not caused by any possible effects of varying substrate availability, high cell density, plasmid replication or the presence of the inducing agent. We hypothesize that the integration of the recombinant protein into the cell membrane physically disrupted the functionality of the efflux pumps, thus resulting in EB-staining of the recombinant cells. This method enabled us to detect changes in the physiological state of single cells 2-4 h before other indications of partial cell damage, such as unbalanced growth, acetate accumulation and an increased CO(2) production rate, were observed. This method therefore shows promise with respect to the further development of an early-warning system to prevent sudden productivity decreases in processes with recombinant E. coli expressing heterologous membrane proteins. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/11467 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Looser, V., Hammes, F., Keller, M., Berney, M., Kovar, K., & Egli, T. (2005). Flow-cytometric detection of changes in the physiological state of E. coli expressing a heterologous membrane protein during carbon-limited fedbatch cultivation. Biotechnology & Bioengineering, 92(1), 69–78. https://doi.org/10.1002/bit.20575
Looser, V. et al. (2005) ‘Flow-cytometric detection of changes in the physiological state of E. coli expressing a heterologous membrane protein during carbon-limited fedbatch cultivation’, Biotechnology & Bioengineering, 92(1), pp. 69–78. Available at: https://doi.org/10.1002/bit.20575.
V. Looser, F. Hammes, M. Keller, M. Berney, K. Kovar, and T. Egli, “Flow-cytometric detection of changes in the physiological state of E. coli expressing a heterologous membrane protein during carbon-limited fedbatch cultivation,” Biotechnology & Bioengineering, vol. 92, no. 1, pp. 69–78, Oct. 2005, doi: 10.1002/bit.20575.
LOOSER, Verena, Frederik HAMMES, Markus KELLER, Michael BERNEY, Karin KOVAR und Thomas EGLI, 2005. Flow-cytometric detection of changes in the physiological state of E. coli expressing a heterologous membrane protein during carbon-limited fedbatch cultivation. Biotechnology & Bioengineering. 5 Oktober 2005. Bd. 92, Nr. 1, S. 69–78. DOI 10.1002/bit.20575
Looser, Verena, Frederik Hammes, Markus Keller, Michael Berney, Karin Kovar, and Thomas Egli. 2005. “Flow-Cytometric Detection of Changes in the Physiological State of E. Coli Expressing a Heterologous Membrane Protein during Carbon-Limited Fedbatch Cultivation.” Biotechnology & Bioengineering 92 (1): 69–78. https://doi.org/10.1002/bit.20575.
Looser, Verena, et al. “Flow-Cytometric Detection of Changes in the Physiological State of E. Coli Expressing a Heterologous Membrane Protein during Carbon-Limited Fedbatch Cultivation.” Biotechnology & Bioengineering, vol. 92, no. 1, Oct. 2005, pp. 69–78, https://doi.org/10.1002/bit.20575.
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