| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Combined use of fluorescent dyes and flow cytometry to quantify the physiological state of Pichia pastoris during the production of heterologous proteins in high-cell-density fed-batch cultures |
| Autor/-in: | Hyka, Petr Züllig, Thomas Ruth, Claudia Looser, Verena Meier, Christian Klein, Joachim Melzoch, Karel Meyer, Hans-Peter Glieder, Anton Kovar, Karin |
| DOI: | 10.1128/AEM.02475-09 |
| Erschienen in: | Applied and Environmental Microbiology |
| Band(Heft): | 76 |
| Heft: | 13 |
| Seite(n): | 4486 |
| Seiten bis: | 4496 |
| Erscheinungsdatum: | 4-Mai-2010 |
| Verlag / Hrsg. Institution: | American Society for Microbiology |
| ISSN: | 0099-2240 1098-5336 |
| Sprache: | Englisch |
| Schlagwörter: | Pichia pastoris |
| Fachgebiet (DDC): | 570: Biologie |
| Zusammenfassung: | Matching both the construction of a recombinant strain and the process design with the characteristics of the target protein has the potential to significantly enhance bioprocess performance, robustness, and reproducibility. The factors affecting the physiological state of recombinant Pichia pastoris Mut+ (methanol utilization-positive) strains and their cell membranes were quantified at the individual cell level using a combination of staining with fluorescent dyes and flow cytometric enumeration. Cell vitalities were found to range from 5 to 95% under various process conditions in high-cell-density fed-batch cultures, with strains producing either porcine trypsinogen or horseradish peroxidase extracellularly. Impaired cell vitality was observed to be the combined effect of production of recombinant protein, low pH, and high cell density. Vitality improved when any one of these stress factors was excluded. At a pH value of 4, which is commonly applied to counter proteolysis, recombinant strains exhibited severe physiological stress, whereas strains without heterologous genes were not affected. Physiologically compromised cells were also found to be increasingly sensitive to methanol when it accumulated in the culture broth. The magnitude of the response varied when different reporters were combined with either the native AOX1 promoter or its d6* variant, which differ in both strength and regulation. Finally, the quantitative assessment of the physiology of individual cells enables the implementation of innovative concepts in bioprocess development. Such concepts are in contrast to the frequently used paradigm, which always assumes a uniform cell population, because differentiation between the individual cells is not possible with methods commonly used. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/10882 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
Dateien zu dieser Ressource:
Es gibt keine Dateien zu dieser Ressource.
Zur Langanzeige
Hyka, P., Züllig, T., Ruth, C., Looser, V., Meier, C., Klein, J., Melzoch, K., Meyer, H.-P., Glieder, A., & Kovar, K. (2010). Combined use of fluorescent dyes and flow cytometry to quantify the physiological state of Pichia pastoris during the production of heterologous proteins in high-cell-density fed-batch cultures. Applied and Environmental Microbiology, 76(13), 4486–4496. https://doi.org/10.1128/AEM.02475-09
Hyka, P. et al. (2010) ‘Combined use of fluorescent dyes and flow cytometry to quantify the physiological state of Pichia pastoris during the production of heterologous proteins in high-cell-density fed-batch cultures’, Applied and Environmental Microbiology, 76(13), pp. 4486–4496. Available at: https://doi.org/10.1128/AEM.02475-09.
P. Hyka et al., “Combined use of fluorescent dyes and flow cytometry to quantify the physiological state of Pichia pastoris during the production of heterologous proteins in high-cell-density fed-batch cultures,” Applied and Environmental Microbiology, vol. 76, no. 13, pp. 4486–4496, May 2010, doi: 10.1128/AEM.02475-09.
HYKA, Petr, Thomas ZÜLLIG, Claudia RUTH, Verena LOOSER, Christian MEIER, Joachim KLEIN, Karel MELZOCH, Hans-Peter MEYER, Anton GLIEDER und Karin KOVAR, 2010. Combined use of fluorescent dyes and flow cytometry to quantify the physiological state of Pichia pastoris during the production of heterologous proteins in high-cell-density fed-batch cultures. Applied and Environmental Microbiology. 4 Mai 2010. Bd. 76, Nr. 13, S. 4486–4496. DOI 10.1128/AEM.02475-09
Hyka, Petr, Thomas Züllig, Claudia Ruth, Verena Looser, Christian Meier, Joachim Klein, Karel Melzoch, Hans-Peter Meyer, Anton Glieder, and Karin Kovar. 2010. “Combined Use of Fluorescent Dyes and Flow Cytometry to Quantify the Physiological State of Pichia Pastoris during the Production of Heterologous Proteins in High-Cell-Density Fed-Batch Cultures.” Applied and Environmental Microbiology 76 (13): 4486–96. https://doi.org/10.1128/AEM.02475-09.
Hyka, Petr, et al. “Combined Use of Fluorescent Dyes and Flow Cytometry to Quantify the Physiological State of Pichia Pastoris during the Production of Heterologous Proteins in High-Cell-Density Fed-Batch Cultures.” Applied and Environmental Microbiology, vol. 76, no. 13, May 2010, pp. 4486–96, https://doi.org/10.1128/AEM.02475-09.
Alle Ressourcen in diesem Repository sind urheberrechtlich geschützt, soweit nicht anderweitig angezeigt.