Iterative approach to computational enzyme design

Privett, H. K.; Kiss, G.; Lee, T. M.; Blomberg, R.; Chica, R. A.; Thomas, L. M.; Hilvert, D.; Houk, K. N.; Mayo, S. L.

doi:10.1073/pnas.1118082108

Publication type:	Article in scientific journal
Type of review:	Peer review (publication)
Title:	Iterative approach to computational enzyme design
Authors:	Privett, H. K. Kiss, G. Lee, T. M. Blomberg, R. Chica, R. A. Thomas, L. M. Hilvert, D. Houk, K. N. Mayo, S. L.
DOI:	10.1073/pnas.1118082108
Published in:	Proceedings of the National Academy of Sciences of the United States of America
Volume(Issue):	109
Issue:	10
Page(s):	3790
Pages to:	3795
Issue Date:	2012
Publisher / Ed. Institution:	National Academy of Sciences
ISSN:	0027-8424 1091-6490
Language:	English
Subjects:	Algorithms; Catalysis; Catalytic domain; Computational biology; X-ray crystallography; Ligands; Chemical models; Molecular cnformation; Molecular dynamics simulation; Point mutation; Protein engineering; Protons
Subject (DDC):	660.6: Biotechnology
Abstract:	A general approach for the computational design of enzymes to catalyze arbitrary reactions is a goal at the forefront of the field of protein design. Recently, computationally designed enzymes have been produced for three chemical reactions through the synthesis and screening of a large number of variants. Here, we present an iterative approach that has led to the development of the most catalytically efficient computationally designed enzyme for the Kemp elimination to date. Previously established computational techniques were used to generate an initial design, HG-1, which was catalytically inactive. Analysis of HG-1 with molecular dynamics simulations (MD) and X-ray crystallography indicated that the inactivity might be due to bound waters and high flexibility of residues within the active site. This analysis guided changes to our design procedure, moved the design deeper into the interior of the protein, and resulted in an active Kemp eliminase, HG-2. The cocrystal structure of this enzyme with a transition state analog (TSA) revealed that the TSA was bound in the active site, interacted with the intended catalytic base in a catalytically relevant manner, but was flipped relative to the design model. MD analysis of HG-2 led to an additional point mutation, HG-3, that produced a further threefold improvement in activity. This iterative approach to computational enzyme design, including detailed MD and structural analysis of both active and inactive designs, promises a more complete understanding of the underlying principles of enzymatic catalysis and furthers progress toward reliably producing active enzymes.
URI:	https://digitalcollection.zhaw.ch/handle/11475/9664
Fulltext version:	Published version
License (according to publishing contract):	Licence according to publishing contract
Departement:	Life Sciences and Facility Management
Appears in collections:	Publikationen Life Sciences und Facility Management

Files in This Item:

There are no files associated with this item.

Show full item record

Privett, H. K., Kiss, G., Lee, T. M., Blomberg, R., Chica, R. A., Thomas, L. M., Hilvert, D., Houk, K. N., & Mayo, S. L. (2012). Iterative approach to computational enzyme design. Proceedings of the National Academy of Sciences of the United States of America, 109(10), 3790–3795. https://doi.org/10.1073/pnas.1118082108

Privett, H.K. et al. (2012) ‘Iterative approach to computational enzyme design’, Proceedings of the National Academy of Sciences of the United States of America, 109(10), pp. 3790–3795. Available at: https://doi.org/10.1073/pnas.1118082108.

H. K. Privett et al., “Iterative approach to computational enzyme design,” Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 10, pp. 3790–3795, 2012, doi: 10.1073/pnas.1118082108.

PRIVETT, H. K., G. KISS, T. M. LEE, R. BLOMBERG, R. A. CHICA, L. M. THOMAS, D. HILVERT, K. N. HOUK und S. L. MAYO, 2012. Iterative approach to computational enzyme design. Proceedings of the National Academy of Sciences of the United States of America. 2012. Bd. 109, Nr. 10, S. 3790–3795. DOI 10.1073/pnas.1118082108

Privett, H. K., G. Kiss, T. M. Lee, R. Blomberg, R. A. Chica, L. M. Thomas, D. Hilvert, K. N. Houk, and S. L. Mayo. 2012. “Iterative Approach to Computational Enzyme Design.” Proceedings of the National Academy of Sciences of the United States of America 109 (10): 3790–95. https://doi.org/10.1073/pnas.1118082108.

Privett, H. K., et al. “Iterative Approach to Computational Enzyme Design.” Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 10, 2012, pp. 3790–95, https://doi.org/10.1073/pnas.1118082108.