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dc.contributor.authorDewavrin, Jean-Yves-
dc.contributor.authorHamzavi, Nader-
dc.contributor.authorShim, V.P.W.-
dc.contributor.authorRaghunath, Michael-
dc.date.accessioned2018-08-24T12:44:04Z-
dc.date.available2018-08-24T12:44:04Z-
dc.date.issued2014-
dc.identifier.issn1742-7061de_CH
dc.identifier.issn1878-7568de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/9610-
dc.description.abstractMacromolecular crowding is an optimal physiological feature in intracellular and extracellular spaces, and results from a variety of macromolecules occupying space and contributing to a fractional volume occupancy. Here, we show that soft collagen hydrogels assembled in nature-inspired crowded conditions feature enhanced biophysical properties. We demonstrate that crowding tunes the rate of collagen nucleation and fiber growth, affecting fiber diameter and organization. Adjustments of crowding levels during collagen assembly tune the gel pore size, protein permeability, transparency and resistance to enzymatic degradation. Furthermore, gels assembled in crowded conditions are twice as resistant to mechanical stress as the controls, inducing a 70% boost of proliferation of stem cells cultured on tuned hydrogels. Emulating the crowdedness of interstitial fluids therefore represents a way to optimize the properties of soft collagen gels, with promising applications in soft biomaterials design.de_CH
dc.language.isoende_CH
dc.publisherElsevierde_CH
dc.relation.ispartofActa Biomaterialiade_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectBiomaterialde_CH
dc.subjectExcluded-volume effectde_CH
dc.subjectFibrillogenesisde_CH
dc.subjectHydrogelde_CH
dc.subjectCultured cellsde_CH
dc.subjectCollagen type Ide_CH
dc.subjectHydrogelde_CH
dc.subjectMesenchymal stromal cellsde_CH
dc.subjectPorosityde_CH
dc.subject.ddc660: Technische Chemiede_CH
dc.titleTuning the architecture of three-dimensional collagen hydrogels by physiological macromolecular crowdingde_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementLife Sciences und Facility Managementde_CH
zhaw.organisationalunitInstitut für Chemie und Biotechnologie (ICBT)de_CH
dc.identifier.doi10.1016/j.actbio.2014.06.006de_CH
dc.identifier.pmid24932771de_CH
zhaw.funding.euNode_CH
zhaw.issue10de_CH
zhaw.originated.zhawNode_CH
zhaw.pages.end4359de_CH
zhaw.pages.start4351de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume10de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
zhaw.webfeedMetabolic Tissue Engineeringde_CH
Appears in collections:Publikationen Life Sciences und Facility Management

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