| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Strategy for allergenicity assessment of "natural novel foods" : clinical and molecular investigation of exotic vegetables (water spinach, hyacinth bean and Ethiopian eggplant) |
| Autor/-in: | Gubesch, Michaela Theler, Barbara Mathis, Alex Dutta, Manjula Baumer, Beatrice Holzhauser, Thomas Vieths, Stefan Ballmer-Weber, Barbara K. |
| DOI: | 10.1111/j.1398-9995.2007.01474.x |
| Erschienen in: | Allergy |
| Band(Heft): | 62 |
| Heft: | 11 |
| Seite(n): | 1243 |
| Seiten bis: | 1250 |
| Erscheinungsdatum: | 2007 |
| Verlag / Hrsg. Institution: | Wiley |
| ISSN: | 0105-4538 1398-9995 |
| Sprache: | Englisch |
| Schlagwörter: | Food; Potential; Allergenic; Allergy |
| Fachgebiet (DDC): | 610: Medizin und Gesundheit |
| Zusammenfassung: | Background: Foods not commonly consumed in the European Union (EU) must be proven save before brought to market, including an assessment of allergenicity. We present a three-stepwise strategy for allergenicity assessment of natural novel foods using three novel vegetables as example (water spinach, hyacinth bean, Ethiopian eggplant). Methods: First, vegetable extracts were analysed for the presence of pan-allergens [Bet v 1 homologous proteins, profilins, non specific lipid transfer proteins (LTP)] by immunoblot analysis with specific animal antibodies. Second, the IgE-binding of the food extracts was investigated by EAST (Enzyme-allergosorbent test) and immunoblot analysis using sera with IgE-reactivity to known pan-allergens or to phylogenetically related foods from subjects 1) allergic to birch, grass and mugwort pollen, 2) with food allergy to soy, peanut, tomato, multiple pollen-related foods and 3) sensitised to LTP. Third, the clinical relevance of IgE-binding was assessed in vivo by skin prick testing (SPT) and open oral food challenges (OFC). Results: Profilin and LTP were detected by animal antibodies in all vegetables, a Bet v 1 homologue selectively in hyacinth bean. IgE-binding to LTP, profilin and a Bet v 1 homologue was proven by immunoblot analysis and EAST. Positive SPT and OFC results were observed for all vegetables in pollen-allergic patients. Conclusion: Our stepwise procedure confirmed the presence and IgE-binding capacity of novel vegetable proteins homologous to known allergens in endemic vegetable foods. In vivo testing proved the potential of the novel vegetables to elicit clinical allergy. Hence, our described algorithm seems to be applicable for allergenicity testing of natural novel foods. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/9436 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Lebensmittel- und Getränkeinnovation (ILGI) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Gubesch, M., Theler, B., Mathis, A., Dutta, M., Baumer, B., Holzhauser, T., Vieths, S., & Ballmer-Weber, B. K. (2007). Strategy for allergenicity assessment of “natural novel foods” : clinical and molecular investigation of exotic vegetables (water spinach, hyacinth bean and Ethiopian eggplant). Allergy, 62(11), 1243–1250. https://doi.org/10.1111/j.1398-9995.2007.01474.x
Gubesch, M. et al. (2007) ‘Strategy for allergenicity assessment of ‘natural novel foods’ : clinical and molecular investigation of exotic vegetables (water spinach, hyacinth bean and Ethiopian eggplant)’, Allergy, 62(11), pp. 1243–1250. Available at: https://doi.org/10.1111/j.1398-9995.2007.01474.x.
M. Gubesch et al., “Strategy for allergenicity assessment of “natural novel foods” : clinical and molecular investigation of exotic vegetables (water spinach, hyacinth bean and Ethiopian eggplant),” Allergy, vol. 62, no. 11, pp. 1243–1250, 2007, doi: 10.1111/j.1398-9995.2007.01474.x.
GUBESCH, Michaela, Barbara THELER, Alex MATHIS, Manjula DUTTA, Beatrice BAUMER, Thomas HOLZHAUSER, Stefan VIETHS und Barbara K. BALLMER-WEBER, 2007. Strategy for allergenicity assessment of „natural novel foods“ : clinical and molecular investigation of exotic vegetables (water spinach, hyacinth bean and Ethiopian eggplant). Allergy. 2007. Bd. 62, Nr. 11, S. 1243–1250. DOI 10.1111/j.1398-9995.2007.01474.x
Gubesch, Michaela, Barbara Theler, Alex Mathis, Manjula Dutta, Beatrice Baumer, Thomas Holzhauser, Stefan Vieths, and Barbara K. Ballmer-Weber. 2007. “Strategy for Allergenicity Assessment of “Natural Novel Foods” : Clinical and Molecular Investigation of Exotic Vegetables (Water Spinach, Hyacinth Bean and Ethiopian Eggplant).” Allergy 62 (11): 1243–50. https://doi.org/10.1111/j.1398-9995.2007.01474.x.
Gubesch, Michaela, et al. “Strategy for Allergenicity Assessment of “Natural Novel Foods” : Clinical and Molecular Investigation of Exotic Vegetables (Water Spinach, Hyacinth Bean and Ethiopian Eggplant).” Allergy, vol. 62, no. 11, 2007, pp. 1243–50, https://doi.org/10.1111/j.1398-9995.2007.01474.x.
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