Simple and rapid sample preparation for 25OH-Vitamin D3/D2 using an immobilized liquid

Weibel, Roland; Zaborosch, Christiane; Svoboda, Michal; Geyer, Roland; Bartl, Ralf

Publication type:	Conference poster
Type of review:	No review
Title:	Simple and rapid sample preparation for 25OH-Vitamin D3/D2 using an immobilized liquid
Authors:	Weibel, Roland Zaborosch, Christiane Svoboda, Michal Geyer, Roland Bartl, Ralf
Conference details:	MSACL Mass Spectrometry Applications to the Clinical Lab, San Diego, USA, 5-9 February 2011
Issue Date:	Feb-2011
Language:	English
Subjects:	Diagnostic; Sample preparation; Mass spectrometry; Vitamin D
Subject (DDC):	540: Chemistry
Abstract:	The metabolites 25OH-Vitamin D3/D2 represent the storage form of Vitamin D for the human body. Healthy subjects should display concentrations between 30-70 ng/mL in plasma while a level below 30 ng/mL indicates Vitamin D deficiency. Monitoring 25OH-Vit D3/D2 e.g. by LC-MS/MS is crucial for the detection of those deficiencies which result in severe health threats if not treated in a timely manner. Prior to any analysis by LC-MS/MS, however, most of the matrix – which means proteins, lipids, carbohydrates, salts and others – has to be removed from biological samples like serum or plasma. Extraction using an immobilized liquid is a rapid and simple method to produce sample extracts that are sufficiently matrix-depleted to be directly injected onto an analytical column for quantification by LCMS/MS. In addition, this sample preparation procedure bears a promising potential for straight forward automation.
URI:	https://digitalcollection.zhaw.ch/handle/11475/9213
Fulltext version:	Published version
License (according to publishing contract):	Not specified
Departement:	Life Sciences and Facility Management
Organisational Unit:	Institute of Chemistry and Biotechnology (ICBT)
Appears in collections:	Publikationen Life Sciences und Facility Management

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Weibel, R., Zaborosch, C., Svoboda, M., Geyer, R., & Bartl, R. (2011, February). Simple and rapid sample preparation for 25OH-Vitamin D3/D2 using an immobilized liquid. MSACL Mass Spectrometry Applications to the Clinical Lab, San Diego, USA, 5-9 February 2011.

Weibel, R. et al. (2011) ‘Simple and rapid sample preparation for 25OH-Vitamin D3/D2 using an immobilized liquid’, in MSACL Mass Spectrometry Applications to the Clinical Lab, San Diego, USA, 5-9 February 2011.

R. Weibel, C. Zaborosch, M. Svoboda, R. Geyer, and R. Bartl, “Simple and rapid sample preparation for 25OH-Vitamin D3/D2 using an immobilized liquid,” in MSACL Mass Spectrometry Applications to the Clinical Lab, San Diego, USA, 5-9 February 2011, Feb. 2011.

WEIBEL, Roland, Christiane ZABOROSCH, Michal SVOBODA, Roland GEYER und Ralf BARTL, 2011. Simple and rapid sample preparation for 25OH-Vitamin D3/D2 using an immobilized liquid. In: MSACL Mass Spectrometry Applications to the Clinical Lab, San Diego, USA, 5-9 February 2011. Conference poster. Februar 2011

Weibel, Roland, Christiane Zaborosch, Michal Svoboda, Roland Geyer, and Ralf Bartl. 2011. “Simple and Rapid Sample Preparation for 25OH-Vitamin D3/D2 Using an Immobilized Liquid.” Conference poster. In MSACL Mass Spectrometry Applications to the Clinical Lab, San Diego, USA, 5-9 February 2011.

Weibel, Roland, et al. “Simple and Rapid Sample Preparation for 25OH-Vitamin D3/D2 Using an Immobilized Liquid.” MSACL Mass Spectrometry Applications to the Clinical Lab, San Diego, USA, 5-9 February 2011, 2011.