| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | New miniature stirred-tank bioreactors for parallel study of enzymatic biomass hydrolysis |
| Autor/-in: | Riedlberger, Peter Weuster-Botz, Dirk |
| DOI: | 10.1016/j.biortech.2011.12.019 |
| Erschienen in: | Bioresource Technology |
| Band(Heft): | 106 |
| Seite(n): | 138 |
| Seiten bis: | 146 |
| Erscheinungsdatum: | 2012 |
| Verlag / Hrsg. Institution: | Elsevier |
| ISSN: | 0960-8524 1873-2976 |
| Sprache: | Englisch |
| Schlagwörter: | Biotechnology; Cellulase; Crystallization; Enzymes; Glucose; Hydrolysis; Miniaturization; Suspensions; Time Factors; Waste Products; Xylose; Beta-Glucosidase; Biomass; Bioreactors; Catalysis |
| Fachgebiet (DDC): | 660: Technische Chemie |
| Zusammenfassung: | Many factors strongly influence the enzymatic hydrolysis of biomass to fermentable sugars (feedstock composition, pretreatment, enzymes and enzyme loading). In order to optimize the reaction conditions for the hydrolysis of biomass, an accurate high-throughput bioprocess development tool is mandatory, which enables a parallelization and an easy scale-up. New S-shaped impellers were developed for magnetically inductive driven stirred-tank bioreactors at a 10 mL-scale. An efficient and reproducible homogenization was shown at 20% w/w solids loading of microcrystalline cellulose and at, 4-10% with wheat straw in 48 parallel operated stirred-tank bioreactors. The scale-up was successfully validated for the enzymatic hydrolysis of wheat straw suspensions and microcrystalline cellulose mixtures by application of a cellulase complex at a milliliter- and liter-scale. As an example, the parallel stirred-tank bioreactor system was applied for the evaluation of enzymatic batch hydrolyses of plant materials with varying pretreatments. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/9147 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Chemie und Biotechnologie (ICBT) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
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Riedlberger, P., & Weuster-Botz, D. (2012). New miniature stirred-tank bioreactors for parallel study of enzymatic biomass hydrolysis. Bioresource Technology, 106, 138–146. https://doi.org/10.1016/j.biortech.2011.12.019
Riedlberger, P. and Weuster-Botz, D. (2012) ‘New miniature stirred-tank bioreactors for parallel study of enzymatic biomass hydrolysis’, Bioresource Technology, 106, pp. 138–146. Available at: https://doi.org/10.1016/j.biortech.2011.12.019.
P. Riedlberger and D. Weuster-Botz, “New miniature stirred-tank bioreactors for parallel study of enzymatic biomass hydrolysis,” Bioresource Technology, vol. 106, pp. 138–146, 2012, doi: 10.1016/j.biortech.2011.12.019.
RIEDLBERGER, Peter und Dirk WEUSTER-BOTZ, 2012. New miniature stirred-tank bioreactors for parallel study of enzymatic biomass hydrolysis. Bioresource Technology. 2012. Bd. 106, S. 138–146. DOI 10.1016/j.biortech.2011.12.019
Riedlberger, Peter, and Dirk Weuster-Botz. 2012. “New Miniature Stirred-Tank Bioreactors for Parallel Study of Enzymatic Biomass Hydrolysis.” Bioresource Technology 106: 138–46. https://doi.org/10.1016/j.biortech.2011.12.019.
Riedlberger, Peter, and Dirk Weuster-Botz. “New Miniature Stirred-Tank Bioreactors for Parallel Study of Enzymatic Biomass Hydrolysis.” Bioresource Technology, vol. 106, 2012, pp. 138–46, https://doi.org/10.1016/j.biortech.2011.12.019.
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