Please use this identifier to cite or link to this item:
|Title:||Molecular investigation of isolates from a multistate polymicrobial outbreak associated with contaminated total parenteral nutrition in Brazil|
|Authors :||Pillonetto, Marcelo|
Gomes, Suzie M. T.
Oliveira, Marluce A. A.
Timm, Loeci N.
Martins, Andreza F.
Barth, Afonso L.
Smits, Theo H. M.
Mira, Marcelo T.
|Published in :||BMC Infectious Diseases|
|Publisher / Ed. Institution :||BioMed Central|
|License (according to publishing contract) :||CC BY 4.0: Attribution 4.0 International|
|Type of review:||Open peer review|
|Subjects :||Acinetobacter baumannii; Rhizobium radiobacter; Rep-PCR; Bacterial identification; Phytobacter diazotrophicus; Pantoea; TPN|
|Subject (DDC) :||570: Biology |
616: Internal medicine and diseases
|Abstract:||Background: Between November 2013 and June 2014, 56 cases of bacteremia (15 deaths) associated with the use of Total Parenteral Nutrition (TPN) and/or calcium gluconate (CG) were reported in four Brazilian states. Methods: We analyzed 73 bacterial isolates from four states: 45 from blood, 25 from TPN and three from CG, originally identified as Acinetobacter baumannii, Rhizobium radiobacter, Pantoea sp. or Enterobacteriaceae using molecular methods. Results: The first two bacterial species were confirmed while the third group of species could not be identified using standard identification protocols. These isolates were subsequently identified by Multi-Locus Sequence Analysis as Phytobacter diazotrophicus, a species related to strains from similar outbreaks in the United States in the 1970’s. Within each species, TPN and blood isolates proved to be clonal, whereas the R. radiobacter isolates retrieved from CG were found to be unrelated. Conclusion: This is the first report of a three-species outbreak caused by TPN contaminated with A. baumannii, R. radiobacter and P. diazotrophicus. The concomitant presence of clonal A. baumannii and P. diazotrophicus isolates in several TPN and blood samples, as well as the case of one patient, where all three different species were isolated simultaneously, suggest that the outbreak may be ascribed to a discrete contamination of TPN. In addition, this study highlights the clinical relevance of P. diazotrophicus, which has been involved in outbreaks in the past, but was often misidentified as P. agglomerans.|
|Departement:||Life Sciences and Facility Management|
|Organisational Unit:||Institute of Natural Resource Sciences (IUNR)|
|Publication type:||Article in scientific Journal|
|Published as part of the ZHAW project :||Sepsis-Associated Microorganisms in Brazilian Ambulatories (SAMBA)|
|Appears in Collections:||Publikationen Life Sciences und Facility Management|
Files in This Item:
|Phytobacter outbreak (Pillonetto BMC2018).pdf||1.89 MB||Adobe PDF|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.