Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-3855
Title: Accounting for programmed ribosomal frameshifting in the computation of codon usage bias indices
Authors : Garcia, Victor
Anisimova, Maria
Publisher / Ed. Institution : Cold Spring Harbor Laboratory
Publisher / Ed. Institution: bioRxiv
Issue Date: 2-Apr-2018
Language : Englisch / English
Subjects : Genetics; Codon usage bias; Programmed ribosomal frameshifting
Subject (DDC) : 572: Biochemie / Biochemistry
Abstract: Experimental evidence shows that synonymous mutations can have important consequences on genetic fitness. Many organisms display codon usage bias (CUB), where synonymous codons that are translated into the same amino acid appear with distinct frequency. CUB is thought to arise from selection for translational efficiency and accuracy, termed the translational efficiency hypothesis (TEH). Indeed, CUB indices correlate with protein expression levels, which is widely interpreted as evidence for translational selection. However, these tests neglect -1 programmed ribosomal frameshifting (-1 PRF), an important translational disruption effect found across all organisms of the tree of life. Genes that contain -1 PRF signals should cost more to express than genes without. Thus, CUB indices that do not consider -1 PRF may overestimate genes' true adaptation to translational efficiency and accuracy constraints. Here, we first investigate whether -1 PRF signals do indeed carry such translational cost. We then propose two corrections for CUB indices for genes containing -1 PRF signals. We retest the TEH under these corrections. We find that the correlation between corrected CUB index and protein expression remains intact for most levels of uniform -1 PRF efficiencies, and tends to increase when these efficiencies decline with protein expression. We conclude that the TEH is strengthened and that -1 PRF events constitute a promising and useful tool to examine the relationships between CUB and selection for translation efficiency and accuracy.
Departement: Life Sciences und Facility Management
Organisational Unit: Institut für Angewandte Simulation (IAS)
Publication type: Weiteres (textuelles Material) / Other (textual)
DOI : https://doi.org/10.1101/293340
10.21256/zhaw-3855
URI: https://digitalcollection.zhaw.ch/handle/11475/7944
License (according to publishing contract) : CC BY-NC-ND 4.0: Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International
Appears in Collections:Publikationen Life Sciences und Facility Management

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