| Publikationstyp: | Beitrag in wissenschaftlicher Zeitschrift |
| Art der Begutachtung: | Peer review (Publikation) |
| Titel: | Molecular analysis of a novel gene cluster encoding an insect toxin in plant-associated strains of Pseudomonas fluorescens |
| Autor/-in: | Péchy-Tarr, M. Bruck, D. J. Maurhofer, M. Fischer, Esther Vogne, C. Henkels, M. D. Donahue, K. M. Grunder, Jürg Loper, J. E. Keel, C. |
| DOI: | 10.1111/j.1462-2920.2008.01662.x |
| Erschienen in: | Environmental Microbiology |
| Band(Heft): | 10 |
| Heft: | 9 |
| Seite(n): | 2368 |
| Seiten bis: | 2386 |
| Erscheinungsdatum: | 2008 |
| Verlag / Hrsg. Institution: | Wiley |
| ISSN: | 1462-2920 1462-2912 |
| Sprache: | Englisch |
| Schlagwörter: | Phytopathology; Bacterial toxin |
| Fachgebiet (DDC): | 630: Landwirtschaft |
| Zusammenfassung: | Pseudomonas fluorescens CHA0 and the related strain Pf-5 are well-characterized representatives of rhizosphere bacteria that have the capacity to protect crop plants from fungal root diseases, mainly by releasing a variety of exoproducts that are toxic to plant pathogenic fungi. Here, we report that the two plant-beneficial pseudomonads also exhibit potent insecticidal activity. Anti-insect activity is linked to a novel genomic locus encoding a large protein toxin termed Fit (for P. fluorescensinsecticidal toxin) that is related to the insect toxin Mcf (Makes caterpillars floppy) of the entomopathogen Photorhabdus luminescens, a mutualist of insect-invading nematodes. When injected into the haemocoel, even low doses of P. fluorescens CHA0 or Pf-5 killed larvae of the tobacco hornworm Manduca sexta and the greater wax moth Galleria mellonella. In contrast, mutants of CHA0 or Pf-5 with deletions in the Fit toxin gene were significantly less virulent to the larvae. When expressed from an inducible promoter in a non-toxic Escherichia coli host, the Fit toxin gene was sufficient to render the bacterium toxic to both insect hosts. Our findings establish the Fit gene products of P. fluorescens CHA0 and Pf-5 as potent insect toxins that define previously unappreciated anti-insect properties of these plant-colonizing bacteria. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/7568 |
| Volltext Version: | Publizierte Version |
| Lizenz (gemäss Verlagsvertrag): | Lizenz gemäss Verlagsvertrag |
| Departement: | Life Sciences und Facility Management |
| Organisationseinheit: | Institut für Umwelt und Natürliche Ressourcen (IUNR) |
| Enthalten in den Sammlungen: | Publikationen Life Sciences und Facility Management |
Dateien zu dieser Ressource:
Es gibt keine Dateien zu dieser Ressource.
Zur Langanzeige
Péchy-Tarr, M., Bruck, D. J., Maurhofer, M., Fischer, E., Vogne, C., Henkels, M. D., Donahue, K. M., Grunder, J., Loper, J. E., & Keel, C. (2008). Molecular analysis of a novel gene cluster encoding an insect toxin in plant-associated strains of Pseudomonas fluorescens. Environmental Microbiology, 10(9), 2368–2386. https://doi.org/10.1111/j.1462-2920.2008.01662.x
Péchy-Tarr, M. et al. (2008) ‘Molecular analysis of a novel gene cluster encoding an insect toxin in plant-associated strains of Pseudomonas fluorescens’, Environmental Microbiology, 10(9), pp. 2368–2386. Available at: https://doi.org/10.1111/j.1462-2920.2008.01662.x.
M. Péchy-Tarr et al., “Molecular analysis of a novel gene cluster encoding an insect toxin in plant-associated strains of Pseudomonas fluorescens,” Environmental Microbiology, vol. 10, no. 9, pp. 2368–2386, 2008, doi: 10.1111/j.1462-2920.2008.01662.x.
PÉCHY-TARR, M., D. J. BRUCK, M. MAURHOFER, Esther FISCHER, C. VOGNE, M. D. HENKELS, K. M. DONAHUE, Jürg GRUNDER, J. E. LOPER und C. KEEL, 2008. Molecular analysis of a novel gene cluster encoding an insect toxin in plant-associated strains of Pseudomonas fluorescens. Environmental Microbiology. 2008. Bd. 10, Nr. 9, S. 2368–2386. DOI 10.1111/j.1462-2920.2008.01662.x
Péchy-Tarr, M., D. J. Bruck, M. Maurhofer, Esther Fischer, C. Vogne, M. D. Henkels, K. M. Donahue, Jürg Grunder, J. E. Loper, and C. Keel. 2008. “Molecular Analysis of a Novel Gene Cluster Encoding an Insect Toxin in Plant-Associated Strains of Pseudomonas Fluorescens.” Environmental Microbiology 10 (9): 2368–86. https://doi.org/10.1111/j.1462-2920.2008.01662.x.
Péchy-Tarr, M., et al. “Molecular Analysis of a Novel Gene Cluster Encoding an Insect Toxin in Plant-Associated Strains of Pseudomonas Fluorescens.” Environmental Microbiology, vol. 10, no. 9, 2008, pp. 2368–86, https://doi.org/10.1111/j.1462-2920.2008.01662.x.
Alle Ressourcen in diesem Repository sind urheberrechtlich geschützt, soweit nicht anderweitig angezeigt.