Genome-based population structure analysis of the strawberry plant pathogen Xanthomonas fragariae reveals two distinct groups that evolved independently before its species description

Gétaz, Michael; Krijger, Marjon; Rezzonico, Fabio; Smits, Theo; van der Wolf, Jan M.; Pothier, Joël F.

doi:10.1099/mgen.0.000189

Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-3746

Full metadata record

DC Field	Value	Language
dc.contributor.author	Gétaz, Michael	-
dc.contributor.author	Krijger, Marjon	-
dc.contributor.author	Rezzonico, Fabio	-
dc.contributor.author	Smits, Theo	-
dc.contributor.author	van der Wolf, Jan M.	-
dc.contributor.author	Pothier, Joël F.	-
dc.date.accessioned	2018-06-18T08:05:57Z	-
dc.date.available	2018-06-18T08:05:57Z	-
dc.date.issued	2018	-
dc.identifier.issn	2057-5858	de_CH
dc.identifier.uri	https://digitalcollection.zhaw.ch/handle/11475/6990	-
dc.description.abstract	Xanthomonas fragariae is a quarantine organism in Europe, causing angular leaf spots on strawberry plants. It is spreading worldwide in strawberry-producing regions due to import of plant material through trade and human activities. In order to resolve the population structure at the strain level, we have employed high-resolution molecular typing tools on a comprehensive strain collection representing global and temporal distribution of the pathogen. Clustered regularly interspaced short palindromic repeat regions (CRISPRs) and variable number of tandem repeats (VNTRs) were identified within the reference genome of X. fragariae LMG 25863 as a potential source of variation. Strains from our collection were whole-genome sequenced and used in order to identify variable spacers and repeats for discriminative purpose. CRISPR spacer analysis and multiple-locus VNTR analysis (MLVA) displayed a congruent population structure, in which two major groups and a total of four subgroups were revealed. The two main groups were genetically separated before the first X. fragariae isolate was described and are potentially responsible for the worldwide expansion of the bacterial disease. Three primer sets were designed for discriminating CRISPR-associated markers in order to streamline group determination of novel isolates. Overall, this study describes typing methods to discriminate strains and monitor the pathogen population structure, more especially in the view of a new outbreak of the pathogen.	de_CH
dc.language.iso	en	de_CH
dc.publisher	Microbiology Society	de_CH
dc.relation.ispartof	Microbial Genomics	de_CH
dc.rights	http://creativecommons.org/licenses/by-nc/4.0/	de_CH
dc.subject.ddc	572: Biochemie	de_CH
dc.subject.ddc	634: Obstanlagen, Früchte und Forstwirtschaft	de_CH
dc.title	Genome-based population structure analysis of the strawberry plant pathogen Xanthomonas fragariae reveals two distinct groups that evolved independently before its species description	de_CH
dc.type	Beitrag in wissenschaftlicher Zeitschrift	de_CH
dcterms.type	Text	de_CH
zhaw.departement	Life Sciences und Facility Management	de_CH
zhaw.organisationalunit	Institut für Umwelt und Natürliche Ressourcen (IUNR)	de_CH
dc.identifier.doi	10.1099/mgen.0.000189	de_CH
dc.identifier.doi	10.21256/zhaw-3746	-
dc.identifier.pmid	29874158	de_CH
zhaw.funding.eu	info:eu-repo/grantAgreement/EC/FP7/613678//Strategies to develop effective, innovative and practical approaches to protect major European fruit crops from pests and pathogens/DROPSA	de_CH
zhaw.issue	7	de_CH
zhaw.originated.zhaw	Yes	de_CH
zhaw.publication.status	acceptedVersion	de_CH
zhaw.volume	4	de_CH
zhaw.publication.review	Peer review (Publikation)	de_CH
zhaw.webfeed	Umweltgenomik	de_CH
zhaw.funding.zhaw	DROPSA: Strategies to develop effective, innovative and practical approaches to protect major European fruit crops from pests and pathogens	de_CH
zhaw.relation.references	https://www.ebi.ac.uk/ena/browser/view/PRJEB25730	de_CH
Appears in collections:	Publikationen Life Sciences und Facility Management

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Gétaz, M., Krijger, M., Rezzonico, F., Smits, T., van der Wolf, J. M., & Pothier, J. F. (2018). Genome-based population structure analysis of the strawberry plant pathogen Xanthomonas fragariae reveals two distinct groups that evolved independently before its species description. Microbial Genomics, 4(7). https://doi.org/10.1099/mgen.0.000189

Gétaz, M. et al. (2018) ‘Genome-based population structure analysis of the strawberry plant pathogen Xanthomonas fragariae reveals two distinct groups that evolved independently before its species description’, Microbial Genomics, 4(7). Available at: https://doi.org/10.1099/mgen.0.000189.

M. Gétaz, M. Krijger, F. Rezzonico, T. Smits, J. M. van der Wolf, and J. F. Pothier, “Genome-based population structure analysis of the strawberry plant pathogen Xanthomonas fragariae reveals two distinct groups that evolved independently before its species description,” Microbial Genomics, vol. 4, no. 7, 2018, doi: 10.1099/mgen.0.000189.

GÉTAZ, Michael, Marjon KRIJGER, Fabio REZZONICO, Theo SMITS, Jan M. VAN DER WOLF und Joël F. POTHIER, 2018. Genome-based population structure analysis of the strawberry plant pathogen Xanthomonas fragariae reveals two distinct groups that evolved independently before its species description. Microbial Genomics. 2018. Bd. 4, Nr. 7. DOI 10.1099/mgen.0.000189

Gétaz, Michael, Marjon Krijger, Fabio Rezzonico, Theo Smits, Jan M. van der Wolf, and Joël F. Pothier. 2018. “Genome-Based Population Structure Analysis of the Strawberry Plant Pathogen Xanthomonas Fragariae Reveals Two Distinct Groups That Evolved Independently before Its Species Description.” Microbial Genomics 4 (7). https://doi.org/10.1099/mgen.0.000189.

Gétaz, Michael, et al. “Genome-Based Population Structure Analysis of the Strawberry Plant Pathogen Xanthomonas Fragariae Reveals Two Distinct Groups That Evolved Independently before Its Species Description.” Microbial Genomics, vol. 4, no. 7, 2018, https://doi.org/10.1099/mgen.0.000189.