1. ZHAW digitalcollection
  2. School of Engineering
  3. Publikationen
  4. Publikationen School of Engineering
Full metadata record
DC FieldValueLanguage
dc.contributor.authorSchweizer, Anja-
dc.contributor.authorRohrer, Jack-
dc.contributor.authorSlot, Jan W.-
dc.contributor.authorGeuze, Hans J.-
dc.contributor.authorKornfeld, Stuart-
dc.date.accessioned2018-06-13T09:45:46Z-
dc.date.available2018-06-13T09:45:46Z-
dc.date.issued1995-
dc.identifier.issn0021-9533de_CH
dc.identifier.urihttp://jcs.biologists.org/content/108/6/2477de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/6837-
dc.description.abstractp63 is a type II integral membrane protein that has previously been suggested to be a resident protein of a membrane network interposed between the ER and the Golgi apparatus. In the present study, we have produced a polyclonal antibody against the purified human p63 protein to reassess the subcellular distribution of p63 by confocal immunofluorescence, immunoelectron microscopy, and cell fractionation. Double immunofluorescence of COS cells showed significant colocalization of p63 and a KDEL-containing lumenal ER marker protein, except for differences in the staining of the outer nuclear membrane. Immunoelectron microscopy of native HepG2 cells and of COS cells transfected with p63 revealed that both endogenous and overexpressed p63 are predominantly localized in the rough ER. While p63 was colocalized with protein disulfide isomerase, an ER marker protein, very little overlap of p63 was found with ERGIC-53, an established marker for the ER-Golgi intermediate compartment. When rough and smooth membranes were prepared from rat liver, p63 was found to copurify with ribophorin II, a rough ER protein. Both p63 and ribophorin II were predominantly recovered in rough microsomes and were largely separated from the intermediate compartment marker protein p58. From these results it is concluded that p63 is localized in the rough ER.de_CH
dc.language.isoende_CH
dc.publisherThe Company of Biologistsde_CH
dc.relation.ispartofJournal of Cell Sciencede_CH
dc.rightsLicence according to publishing contractde_CH
dc.subject.ddc572: Biochemiede_CH
dc.titleReassessment of the subcellular localization of p63de_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementSchool of Engineeringde_CH
zhaw.funding.euNode_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end2485de_CH
zhaw.pages.start2477de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume108de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
Appears in collections:Publikationen School of Engineering

Files in This Item:
There are no files associated with this item.
Show simple item record
Schweizer, A., Rohrer, J., Slot, J. W., Geuze, H. J., & Kornfeld, S. (1995). Reassessment of the subcellular localization of p63. Journal of Cell Science, 108, 2477–2485. http://jcs.biologists.org/content/108/6/2477
Schweizer, A. et al. (1995) ‘Reassessment of the subcellular localization of p63’, Journal of Cell Science, 108, pp. 2477–2485. Available at: http://jcs.biologists.org/content/108/6/2477.
A. Schweizer, J. Rohrer, J. W. Slot, H. J. Geuze, and S. Kornfeld, “Reassessment of the subcellular localization of p63,” Journal of Cell Science, vol. 108, pp. 2477–2485, 1995, [Online]. Available: http://jcs.biologists.org/content/108/6/2477
SCHWEIZER, Anja, Jack ROHRER, Jan W. SLOT, Hans J. GEUZE und Stuart KORNFELD, 1995. Reassessment of the subcellular localization of p63. Journal of Cell Science [online]. 1995. Bd. 108, S. 2477–2485. Verfügbar unter: http://jcs.biologists.org/content/108/6/2477
Schweizer, Anja, Jack Rohrer, Jan W. Slot, Hans J. Geuze, and Stuart Kornfeld. 1995. “Reassessment of the Subcellular Localization of P63.” Journal of Cell Science 108: 2477–85. http://jcs.biologists.org/content/108/6/2477.
Schweizer, Anja, et al. “Reassessment of the Subcellular Localization of P63.” Journal of Cell Science, vol. 108, 1995, pp. 2477–85, http://jcs.biologists.org/content/108/6/2477.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.