| Publication type: | Article in scientific journal |
| Type of review: | Peer review (publication) |
| Title: | Lysosomal hydrolase mannose 6-phosphate uncovering enzyme resides in the trans-Golgi network |
| Authors: | Rohrer, Jack Kornfeld, Rosalind |
| DOI: | 10.1091/mbc.12.6.1623 |
| Published in: | Molecular Biology of the Cell |
| Volume(Issue): | 12 |
| Issue: | 6 |
| Page(s): | 1623 |
| Pages to: | 1631 |
| Issue Date: | Jun-2001 |
| Publisher / Ed. Institution: | American Society for Cell Biology |
| ISSN: | 1939-4586 1059-1524 |
| Language: | English |
| Subject (DDC): | 571: Physiology and related subjects |
| Abstract: | A crucial step in lysosomal biogenesis is catalyzed by "uncovering" enzyme (UCE), which removes a covering N-acetylglucosamine from the mannose 6-phosphate (Man-6-P) recognition marker on lysosomal hydrolases. This study shows that UCE resides in the trans-Golgi network (TGN) and cycles between the TGN and plasma membrane. The cytosolic domain of UCE contains two potential endocytosis motifs: (488)YHPL and C-terminal (511)NPFKD. YHPL is shown to be the more potent of the two in retrieval of UCE from the plasma membrane. A green-fluorescent protein-UCE transmembrane-cytosolic domain fusion protein colocalizes with TGN 46, as does endogenous UCE in HeLa cells, showing that the transmembrane and cytosolic domains determine intracellular location. These data imply that the Man-6-P recognition marker is formed in the TGN, the compartment where Man-6-P receptors bind cargo and are packaged into clathrin-coated vesicles. |
| URI: | https://digitalcollection.zhaw.ch/handle/11475/6801 |
| Fulltext version: | Published version |
| License (according to publishing contract): | Licence according to publishing contract |
| Departement: | Life Sciences and Facility Management |
| Organisational Unit: | Institute of Chemistry and Biotechnology (ICBT) |
| Appears in collections: | Publikationen Life Sciences und Facility Management |
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Rohrer, J., & Kornfeld, R. (2001). Lysosomal hydrolase mannose 6-phosphate uncovering enzyme resides in the trans-Golgi network. Molecular Biology of the Cell, 12(6), 1623–1631. https://doi.org/10.1091/mbc.12.6.1623
Rohrer, J. and Kornfeld, R. (2001) ‘Lysosomal hydrolase mannose 6-phosphate uncovering enzyme resides in the trans-Golgi network’, Molecular Biology of the Cell, 12(6), pp. 1623–1631. Available at: https://doi.org/10.1091/mbc.12.6.1623.
J. Rohrer and R. Kornfeld, “Lysosomal hydrolase mannose 6-phosphate uncovering enzyme resides in the trans-Golgi network,” Molecular Biology of the Cell, vol. 12, no. 6, pp. 1623–1631, Jun. 2001, doi: 10.1091/mbc.12.6.1623.
ROHRER, Jack und Rosalind KORNFELD, 2001. Lysosomal hydrolase mannose 6-phosphate uncovering enzyme resides in the trans-Golgi network. Molecular Biology of the Cell. Juni 2001. Bd. 12, Nr. 6, S. 1623–1631. DOI 10.1091/mbc.12.6.1623
Rohrer, Jack, and Rosalind Kornfeld. 2001. “Lysosomal Hydrolase Mannose 6-Phosphate Uncovering Enzyme Resides in the Trans-Golgi Network.” Molecular Biology of the Cell 12 (6): 1623–31. https://doi.org/10.1091/mbc.12.6.1623.
Rohrer, Jack, and Rosalind Kornfeld. “Lysosomal Hydrolase Mannose 6-Phosphate Uncovering Enzyme Resides in the Trans-Golgi Network.” Molecular Biology of the Cell, vol. 12, no. 6, June 2001, pp. 1623–31, https://doi.org/10.1091/mbc.12.6.1623.
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