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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Schwendimann, Livia Maya | - |
dc.contributor.author | Kauf, Peter | - |
dc.contributor.author | Fieseler, Lars | - |
dc.contributor.author | Gantenbein-Demarchi, Corinne | - |
dc.contributor.author | Miescher Schwenninger, Susanne | - |
dc.date.accessioned | 2018-06-04T13:39:17Z | - |
dc.date.available | 2018-06-04T13:39:17Z | - |
dc.date.issued | 2015-08 | - |
dc.identifier.issn | 0167-7012 | de_CH |
dc.identifier.uri | https://digitalcollection.zhaw.ch/handle/11475/6519 | - |
dc.description.abstract | To monitor dominant species of lactic acid bacteria during cocoa bean fermentation, i.e. Lactobacillus plantarum and Lactobacillus fermentum, a fast and reliable culture-independent qPCR assay was developed. A modified DNA isolation procedure using a commercial kit followed by two species-specific qPCR assays resulted in 100% sensitivity for L. plantarum and L. fermentum. Kruskal–Wallis and post-hoc analyses of data obtained from experiments with cocoa beans that were artificially spiked with decimal concentrations of L. plantarum and L. fermentum strains allowed the calculation of a regression line suitable for the estimation of both species with a detection limit of 3 to 4 Log cells/g cocoa beans. This process was successfully tested for efficacy through the analyses of samples from laboratory-scale cocoa bean fermentations with both the qPCR assay and a culture-dependent method which resulted in comparable results. | de_CH |
dc.language.iso | en | de_CH |
dc.publisher | Elsevier | de_CH |
dc.relation.ispartof | Journal of Microbiological Methods | de_CH |
dc.rights | Licence according to publishing contract | de_CH |
dc.subject | Lactobacillus fermentum | de_CH |
dc.subject | Lactobacillus plantarum | de_CH |
dc.subject | QPCR | de_CH |
dc.subject | Coca bean fermentation | de_CH |
dc.subject.ddc | 664: Lebensmitteltechnologie | de_CH |
dc.title | Development of a quantitative PCR assay for rapid detection of Lactobacillus plantarum and Lactobacillus fermentum in cocoa bean fermentation | de_CH |
dc.type | Beitrag in wissenschaftlicher Zeitschrift | de_CH |
dcterms.type | Text | de_CH |
zhaw.departement | Life Sciences und Facility Management | de_CH |
zhaw.organisationalunit | Institut für Lebensmittel- und Getränkeinnovation (ILGI) | de_CH |
dc.identifier.doi | 10.1016/j.mimet.2015.05.022 | de_CH |
zhaw.funding.eu | No | de_CH |
zhaw.originated.zhaw | Yes | de_CH |
zhaw.pages.end | 99 | de_CH |
zhaw.pages.start | 94 | de_CH |
zhaw.publication.status | publishedVersion | de_CH |
zhaw.volume | 115 | de_CH |
zhaw.publication.review | Peer review (Publikation) | de_CH |
zhaw.webfeed | LM-Biotechnologie | de_CH |
Appears in collections: | Publikationen Life Sciences und Facility Management |
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Schwendimann, L. M., Kauf, P., Fieseler, L., Gantenbein-Demarchi, C., & Miescher Schwenninger, S. (2015). Development of a quantitative PCR assay for rapid detection of Lactobacillus plantarum and Lactobacillus fermentum in cocoa bean fermentation. Journal of Microbiological Methods, 115, 94–99. https://doi.org/10.1016/j.mimet.2015.05.022
Schwendimann, L.M. et al. (2015) ‘Development of a quantitative PCR assay for rapid detection of Lactobacillus plantarum and Lactobacillus fermentum in cocoa bean fermentation’, Journal of Microbiological Methods, 115, pp. 94–99. Available at: https://doi.org/10.1016/j.mimet.2015.05.022.
L. M. Schwendimann, P. Kauf, L. Fieseler, C. Gantenbein-Demarchi, and S. Miescher Schwenninger, “Development of a quantitative PCR assay for rapid detection of Lactobacillus plantarum and Lactobacillus fermentum in cocoa bean fermentation,” Journal of Microbiological Methods, vol. 115, pp. 94–99, Aug. 2015, doi: 10.1016/j.mimet.2015.05.022.
SCHWENDIMANN, Livia Maya, Peter KAUF, Lars FIESELER, Corinne GANTENBEIN-DEMARCHI und Susanne MIESCHER SCHWENNINGER, 2015. Development of a quantitative PCR assay for rapid detection of Lactobacillus plantarum and Lactobacillus fermentum in cocoa bean fermentation. Journal of Microbiological Methods. August 2015. Bd. 115, S. 94–99. DOI 10.1016/j.mimet.2015.05.022
Schwendimann, Livia Maya, Peter Kauf, Lars Fieseler, Corinne Gantenbein-Demarchi, and Susanne Miescher Schwenninger. 2015. “Development of a Quantitative PCR Assay for Rapid Detection of Lactobacillus Plantarum and Lactobacillus Fermentum in Cocoa Bean Fermentation.” Journal of Microbiological Methods 115 (August): 94–99. https://doi.org/10.1016/j.mimet.2015.05.022.
Schwendimann, Livia Maya, et al. “Development of a Quantitative PCR Assay for Rapid Detection of Lactobacillus Plantarum and Lactobacillus Fermentum in Cocoa Bean Fermentation.” Journal of Microbiological Methods, vol. 115, Aug. 2015, pp. 94–99, https://doi.org/10.1016/j.mimet.2015.05.022.
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