Title: Development of a quantitative PCR assay for rapid detection of Lactobacillus plantarum and Lactobacillus fermentum in cocoa bean fermentation
Authors : Schwendimann, Livia Maya
Kauf, Peter
Fieseler, Lars
Gantenbein-Demarchi, Corinne
Miescher Schwenninger, Susanne
Published in : Journal of Microbiological Methods
Volume(Issue) : 115
Pages : 94
Pages to: 99
Publisher / Ed. Institution : Elsevier
Issue Date: Aug-2015
License (according to publishing contract) : Licence according to publishing contract
Type of review: Not specified
Language : English
Subjects : Lactobacillus fermentum; Lactobacillus plantarum; QPCR; Coca bean fermentation
Subject (DDC) : 664: Food technology
Abstract: To monitor dominant species of lactic acid bacteria during cocoa bean fermentation, i.e. Lactobacillus plantarum and Lactobacillus fermentum, a fast and reliable culture-independent qPCR assay was developed. A modified DNA isolation procedure using a commercial kit followed by two species-specific qPCR assays resulted in 100% sensitivity for L. plantarum and L. fermentum. Kruskal–Wallis and post-hoc analyses of data obtained from experiments with cocoa beans that were artificially spiked with decimal concentrations of L. plantarum and L. fermentum strains allowed the calculation of a regression line suitable for the estimation of both species with a detection limit of 3 to 4 Log cells/g cocoa beans. This process was successfully tested for efficacy through the analyses of samples from laboratory-scale cocoa bean fermentations with both the qPCR assay and a culture-dependent method which resulted in comparable results.
Departement: Life Sciences und Facility Management
Organisational Unit: Institute of Food and Beverage Innovation (ILGI)
Publication type: Article in scientific Journal
DOI : 10.1016/j.mimet.2015.05.022
ISSN: 0167-7012
URI: https://digitalcollection.zhaw.ch/handle/11475/6519
Appears in Collections:Publikationen Life Sciences und Facility Management

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