1. ZHAW digitalcollection
  2. School of Engineering
  3. Publikationen
  4. Publikationen School of Engineering
Full metadata record
DC FieldValueLanguage
dc.contributor.authorWachtel, Marco-
dc.contributor.authorDettling, Marcel-
dc.contributor.authorKoscielniak, Eva-
dc.contributor.authorStegmaier, Sabine-
dc.contributor.authorTreuner, Jörn-
dc.contributor.authorSimon-Klingenstein, Katja-
dc.contributor.authorBühlmann, Peter-
dc.contributor.authorNiggli, Felix K.-
dc.contributor.authorSchäfer, Beat W.-
dc.date.accessioned2018-04-03T13:44:39Z-
dc.date.available2018-04-03T13:44:39Z-
dc.date.issued2004-
dc.identifier.issn0008-5472de_CH
dc.identifier.issn0099-7013de_CH
dc.identifier.issn0099-7374de_CH
dc.identifier.issn1538-7445de_CH
dc.identifier.urihttps://digitalcollection.zhaw.ch/handle/11475/4637-
dc.description.abstractRhabdomyosarcoma is a pediatric tumor type, which is classified based on histological criteria into two major subgroups, namely embryonal rhabdomyosarcoma and alveolar rhabdomyosarcoma. The majority, but not all, alveolar rhabdomyosarcoma carry the specific PAX3(7)/FKHR-translocation, whereas there is no consistent genetic abnormality recognized in embryonal rhabdomyosarcoma. To gain additional insight into the genetic characteristics of these subtypes, we used oligonucleotide microarrays to measure the expression profiles of a group of 29 rhabdomyosarcoma biopsy samples (15 embryonal rhabdomyosarcoma, and 10 translocation-positive and 4 translocation-negative alveolar rhabdomyosarcoma). Hierarchical clustering revealed expression signatures clearly discriminating all three of the subgroups. Differentially expressed genes included several tyrosine kinases and G protein-coupled receptors, which might be amenable to pharmacological intervention. In addition, the alveolar rhabdomyosarcoma signature was used to classify an additional alveolar rhabdomyosarcoma case lacking any known PAX3 or PAX7 fusion as belonging to the translocation-positive group, leading to the identification of a novel translocation t(2;2)(q35;p23), which generates a fusion protein composed of PAX3 and the nuclear receptor coactivator NCOA1, having similar transactivation properties as PAX3/FKHR. These experiments demonstrate for the first time that gene expression profiling is capable of identifying novel chromosomal translocations.de_CH
dc.language.isoende_CH
dc.publisherAmerican Association for Cancer Researchde_CH
dc.relation.ispartofCancer Researchde_CH
dc.rightsLicence according to publishing contractde_CH
dc.subjectBase sequencede_CH
dc.subjectChromosomes, human, pair 2de_CH
dc.subjectDNA-binding proteinsde_CH
dc.subjectGene expression profilingde_CH
dc.subjectHistone acetyltransferasesde_CH
dc.subjectHumansde_CH
dc.subjectMolecular sequence datade_CH
dc.subjectNuclear receptor coactivator 1de_CH
dc.subjectOligonucleotide array sequence analysisde_CH
dc.subjectOncogene proteins, fusionde_CH
dc.subjectPAX3 transcription factorde_CH
dc.subjectPaired box transcription factorsde_CH
dc.subjectRhabdomyosarcoma, alveolarde_CH
dc.subjectRhabdomyosarcoma, embryonalde_CH
dc.subjectTrans-activatorsde_CH
dc.subjectTranscription factorsde_CH
dc.subjectTranslocation, geneticde_CH
dc.subject.ddc572: Biochemiede_CH
dc.titleGene expression signatures identify rhabdomyosarcoma subtypes and detect a novel t(2;2)(q35;p23) translocation fusing PAX3 to NCOA1de_CH
dc.typeBeitrag in wissenschaftlicher Zeitschriftde_CH
dcterms.typeTextde_CH
zhaw.departementSchool of Engineeringde_CH
zhaw.publisher.placePhiladelphiade_CH
dc.identifier.doi10.1158/0008-5472.CAN-04-0844de_CH
dc.identifier.pmid15313887de_CH
zhaw.funding.euNode_CH
zhaw.issue16de_CH
zhaw.originated.zhawYesde_CH
zhaw.pages.end5545de_CH
zhaw.pages.start5539de_CH
zhaw.publication.statuspublishedVersionde_CH
zhaw.volume64de_CH
zhaw.publication.reviewPeer review (Publikation)de_CH
Appears in collections:Publikationen School of Engineering

Files in This Item:
There are no files associated with this item.
Show simple item record
Wachtel, M., Dettling, M., Koscielniak, E., Stegmaier, S., Treuner, J., Simon-Klingenstein, K., Bühlmann, P., Niggli, F. K., & Schäfer, B. W. (2004). Gene expression signatures identify rhabdomyosarcoma subtypes and detect a novel t(2;2)(q35;p23) translocation fusing PAX3 to NCOA1. Cancer Research, 64(16), 5539–5545. https://doi.org/10.1158/0008-5472.CAN-04-0844
Wachtel, M. et al. (2004) ‘Gene expression signatures identify rhabdomyosarcoma subtypes and detect a novel t(2;2)(q35;p23) translocation fusing PAX3 to NCOA1’, Cancer Research, 64(16), pp. 5539–5545. Available at: https://doi.org/10.1158/0008-5472.CAN-04-0844.
M. Wachtel et al., “Gene expression signatures identify rhabdomyosarcoma subtypes and detect a novel t(2;2)(q35;p23) translocation fusing PAX3 to NCOA1,” Cancer Research, vol. 64, no. 16, pp. 5539–5545, 2004, doi: 10.1158/0008-5472.CAN-04-0844.
WACHTEL, Marco, Marcel DETTLING, Eva KOSCIELNIAK, Sabine STEGMAIER, Jörn TREUNER, Katja SIMON-KLINGENSTEIN, Peter BÜHLMANN, Felix K. NIGGLI und Beat W. SCHÄFER, 2004. Gene expression signatures identify rhabdomyosarcoma subtypes and detect a novel t(2;2)(q35;p23) translocation fusing PAX3 to NCOA1. Cancer Research. 2004. Bd. 64, Nr. 16, S. 5539–5545. DOI 10.1158/0008-5472.CAN-04-0844
Wachtel, Marco, Marcel Dettling, Eva Koscielniak, Sabine Stegmaier, Jörn Treuner, Katja Simon-Klingenstein, Peter Bühlmann, Felix K. Niggli, and Beat W. Schäfer. 2004. “Gene Expression Signatures Identify Rhabdomyosarcoma Subtypes and Detect a Novel T(2;2)(q35;P23) Translocation Fusing PAX3 to NCOA1.” Cancer Research 64 (16): 5539–45. https://doi.org/10.1158/0008-5472.CAN-04-0844.
Wachtel, Marco, et al. “Gene Expression Signatures Identify Rhabdomyosarcoma Subtypes and Detect a Novel T(2;2)(q35;P23) Translocation Fusing PAX3 to NCOA1.” Cancer Research, vol. 64, no. 16, 2004, pp. 5539–45, https://doi.org/10.1158/0008-5472.CAN-04-0844.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.