Please use this identifier to cite or link to this item: https://doi.org/10.21256/zhaw-3567
Title: Pectobacterium carotovorum subsp. brasiliense and Pectobacterium carotovorum subsp. carotovorum as causal agents of potato soft rot in Algeria
Authors : Naas, Hiba
Sebaihia, Mohammed
Orfei, Benedetta
Rezzonico, Fabio
Buonaurio, Roberto
Moretti, Chiaraluce
Published in : European Journal of Plant Pathology
Publisher / Ed. Institution : Springer
Issue Date: Feb-2018
Language : Englisch / English
Subjects : Algeria; Soft-rotting bacteria; Solanum tuberosum; Pectobacterium; Potato
Subject (DDC) : 570: Biologie
Abstract: During surveys carried out in 2014 and 2015 in Algerian potato fields, severe bacterial tuber soft rot was observed. Twenty-one Gram-negative pectinolytic bacterial isolates were obtained from diseased potato tubers. These induced a hypersensitive reaction and soft rot symptoms when inoculated in tobacco leaves and potato tubers, respectively. PCR amplification using the Y1/Y2 primers demonstrated that all of the bacterial isolates belonged to the genus Pectobacterium. Phylogenetic analysis using partial malate dehydrogenase (mdh) gene sequences revealed that six and 15 isolates grouped with Pectobacterium carotovorum subsp. brasiliense and Pectobacterium carotovorum subsp. carotovorum reference strains, respectively. Multilocus sequence analysis of concatenated partial sequences of the acnA, atpD, gyrB and infB genes carried out on selected bacterial isolates confirmed the results obtained with the mdh gene sequences. PCR amplification with the BR1f/L1r primers that are specific for P. carotovorum subsp. brasiliense confirmed that six of the Algerian isolates belonged to this taxon.
Further description : Erworben im Rahmen der Schweizer Nationallizenzen (http://www.nationallizenzen.ch)
Departement: Life Sciences und Facility Management
Organisational Unit: Institut für Umwelt und Natürliche Ressourcen (IUNR)
Publication type: Beitrag in wissenschaftlicher Zeitschrift / Article in scientific Journal
Type of review: Peer review (Publikation)
DOI : 10.1007/s10658-018-1438-3
10.21256/zhaw-3567
ISSN: 0929-1873
1573-8469
URI: https://digitalcollection.zhaw.ch/handle/11475/4423
License (according to publishing contract) : Lizenz gemäss Verlagsvertrag / Licence according to publishing contract
Restricted until : 2024-01-01
Appears in Collections:Publikationen Life Sciences und Facility Management

Files in This Item:
File Description SizeFormat 
10.1007_s10658-018-1438-3.pdf
  Until 2024-01-01
458.56 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.